Asparagine-Linked Glycosylation 1, beta-1,4-Mannosyltransferase Homolog (S. Cerevisiae) (ALG1) (N-Term) antibody

Details for Product No. ABIN311183
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Synonyms CDG1K, HMAT1, HMT-1, HMT1, MT-1, Mat-1, hMat-1, zgc:66221, wu:fi34b12
(2), (2), (1), (1), (1)
Mouse (Murine), Rat (Rattus), Human
(13), (3), (3)
(12), (2)
Western Blotting (WB)
(14), (6), (5), (2), (1)
Pubmed 1 reference available
Catalog no. ABIN311183
Quantity 50 µg
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Immunogen Synthetic peptide directed towards the N terminal of human ALG1
Predicted Reactivity Human : 100 %, Mouse : 80 %, Rabbit : 70 %, Rat : 70 %
Characteristics This is a rabbit polyclonal antibody against ALG1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ALG1
Background ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K).The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.
Molecular Weight 52 kDa
Gene ID 56052
NCBI Accession NM_019109, NP_061982
UniProt Q9BT22
Research Area Cardiovascular, Fatty Acids, Metabolism, Amino Acids
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 464 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Asparagine-Linked Glycosylation 1, beta-1,4-Mannosyltransferase Homolog (S. Cerevisiae) (ALG1) (N-Term) antibody WB Suggested Anti-ALG1 Antibody Titration: 0.2-1 ug/ml
Positive Control: NTERA2 cell lysate
Background publications Grubenmann, Frank, Hülsmeier et al.: "Deficiency of the first mannosylation step in the N-glycosylation pathway causes congenital disorder of glycosylation type Ik." in: Human molecular genetics, Vol. 13, Issue 5, pp. 535-42, 2004 (PubMed).

Hosts (12), (2)
Reactivities (13), (3), (3)
Applications (14), (6), (5), (2), (1)
Epitopes (2), (2), (1), (1), (1)
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