CD16 antibody (PE)

Catalog No. ABIN316537

Product Details anti-CD16 Antibody (PE)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD16, a 50-65 kDa cell surface molecule. Studies show that the DJ130c antibody recognises an epitope in the first membrane-distal domain of CD16 and recognises both CD16A and CD16B.

Characteristics: Synonyms: IgG Fc receptor III-2, Fc-gamma RIII-alpha, FcR-10, CD16a, FCGR3A, FCG3, IGFR3, Fc-gammaRIIIa, FcRIIIa, Fc-gamma RIII, Low affinity immunoglobulin gamma Fc region receptor III-A

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: CD34 antigen

Clone: DJ130c

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Background: CD16 is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages, whereas CD16B is primarily expressed by granulocytes. In addition, CD16B exists as two allelic variants NA1 and NA2. DJ130c recognises all polymorphonuclear cells irrespective on their NA phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process