HA-Tag antibody

Details for Product No. ABIN318728
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Antigen
Reactivity
Tag
(183), (22), (9), (7), (5), (3), (2), (2), (1)
Host
Rabbit
(98), (93), (19), (7), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(22), (13), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(158), (71), (60), (50), (30), (27), (18), (15), (10), (9), (6), (5), (4), (4), (3), (2), (2), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN318728
632.50 $
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Immunogen Keyhole limpet hemocyanin conjugated epitope tag peptide (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
Sequence YPYDVPDYA
Isotype IgG
Specificity This antibody is specific for the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. The antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts this antibody has been shown not to cross-react with any endogenous proteins.
Purification Purified
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Notes Immunohistochmemistry on paraffin sections (Requires antigen retrieval using heattreatment prior to staining of paraffin embedded sections. Citrate buffer is recommendedfor this purpose). ELISA. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.02M Potassium phosphate, 0.15M Sodium chloride pH7.2, 0.01% Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

Wilson, Niman, Houghten et al.: "The structure of an antigenic determinant in a protein." in: Cell, Vol. 37, Issue 3, pp. 767-78, 1984 (PubMed).

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