HA-Tag antibody (HRP)

Catalog No. ABIN387712

This Mouse Monoclonal antibody specifically detects HA-Tag in WB, ELISA and IP. It has been mentioned in 1 publication

Quick Overview for HA-Tag antibody (HRP) (ABIN387712)

Target: HA-Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 5E11D8

Product Details anti-HA-Tag Antibody (HRP)

Sequence: YPYDVPDYA

Specificity: THE^TM HA Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal and internal HA tagged fusion proteins.

Characteristics: The antibody is suitable for detecting the expression level of HA in fusion proteins. Do not add azide as it inhibits HRP activity.

Purification: Protein A affinity column

Immunogen: A synthetic peptide (YPYDVPDYA) coupled - KLH

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-0.5 µg/mL
Western blot: 0.1-0.5 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing cycles.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-HA-Tag antibody (HRP) (ABIN387712)

Haac, Anderson, Eggleston, Myles, Adelman: "The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes." in: Nucleic acids research, Vol. 43, Issue 7, pp. 3688-700, (2015) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA Tag

Target Type: Tag