HA-Tag antibody

Details for Product No. ABIN319423
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
All Species
(196), (12), (8), (6), (5), (3), (2), (2), (1)
Host
Rabbit
(99), (93), (19), (7), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(22), (13), (10), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(154), (71), (61), (50), (30), (27), (18), (16), (10), (9), (6), (5), (5), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN319423
434.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen The antiserum was produced by immunizing rabbit with synthesized peptide containing the Influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
Specificity This polyclonal antibody detects HA-tagged proteins overexpressed in cells. It recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected cells.
Purification Immunoaffinity Chromatography using epitope-specific peptide.
Alternative Name HA Epitope Tag (YPYDVPDYA)
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Notes Western Blot (1/500-1/1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50% Glycerol 0.02% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-HA-Tag antibody anti-HA-Tag antibody
Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

Validation Images
Did you look for something else?
back to top