CD90 antibody
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- Target See all CD90 (THY1) Antibodies
- CD90 (THY1)
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Reactivity
- Mouse, Rat, Guinea Pig, Rabbit
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD90 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- Specificity
- This antibody recognises CD90, also known as Thy1.1. Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. Clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic neurons, using fluorescence microscopy. This clone has been reported to induce glomerular nephritis in Wistar rats (8). Aff. Const. of the Fab' of MRC OX-7 for: Rat: 3 x 10e9m-1 Mouse: 3 x 10e8m-1
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- Rat Thy1 antigen. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- Clone
- OX-7
- Isotype
- IgG1
- Top Product
- Discover our top product THY1 Primary Antibody
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- Application Notes
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Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD90 (THY1)
- Alternative Name
- CD90 (THY1 Products)
- Background
- Thy1.1 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, T lymphocytes (mouse), immature B cells (rat) and connective tissues.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
- Gene ID
- 21838
- NCBI Accession
- NP_033408
- UniProt
- P01831
- Pathways
- Cell-Cell Junction Organization
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