CD52 antibody (FITC)

Catalog No. ABIN320172

Product Details anti-CD52 Antibody (FITC)

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Clone: YTH34-5

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (Neat): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % Bovine Serum Albumin as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1 (CD52 Products)

Background: The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

NCBI Accession: NP_001794

UniProt: P31358