CCL13 antibody

Catalog No. ABIN328356

Product Details anti-CCL13 Antibody

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Functional Studies (Func), Flow Cytometry (FACS)

Specificity: Recognizes human CCL13. In sandwich ELISAs, less than 1 % cross-reactivity was observed with rhMCP-1 and no significant cross-reactivity or interference was observed with rhMCP-2, rhMCP-3, r  mMARC, r  mMCP-5, rmJE, rhEotaxin, rhEotaxin-2, rmEotaxin, rhRANTES or rmRANTES.

Purification: Protein G purified

Immunogen: E. coli-derived rhCCL13.

Type of Immunogen: Recombinant protein

Clone: 73506

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, Func, Neut

Usage: Neutralization of Bioactivity, Flow Cytometry, ELISA capture. Neutralization of Human CCL13 Bioactivity: The exact concentration of antibody required to neutralize human CCL13 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Flow Cytometry: This antibody was validated for flow cytometry using IL-1 treated A549 cells. For intracellular staining to detect human CCL13, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. ELISA Capture: This antibody can be used as a capture antibody in a human CCL13 ELISA in combination with the biotinylated polyclonal detection antibody. A general protocol is provided on the next page. Using plates coated with 100 μL/well of the capture antibody at 2 μg/mL, in combination with 100 μL/well of the detection antibody, an ELISA for sample volumes of 100 μL can be obtained. To arrive at the optimal dose range for this ELISA, set up a two-fold dilution series of the protein standard starting with 0.5 ng/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: CCL13 / MCP-4 (CCL13 Products)

Background: Name/Gene ID: CCL13
Family: Intercrine

Synonyms: CCL13, C-C motif chemokine 13, CKb10, MCP-4, MCP4, Monocyte chemotactic protein 4, New CC chemokine 1, Small-inducible cytokine A13, NCC1, CK-beta-10, NCC-1, SCYA13

Gene ID: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection