Dachshund Homolog 1 (Drosophila) (DACH1) (Internal Region) antibody

Details for Product No. ABIN334382
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Antigen
Synonyms dach, zgc:158869, AI182278, Dac, Dach, E130112M23Rik, DACH
Epitope
Internal Region
(7), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(38), (18), (16), (13), (12), (12), (12), (1), (1)
Host
Goat
(34), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(29), (10), (10), (5), (4), (3), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334382
374.27 $
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Immunogen Peptide with sequence SPVENTPQNNECK, from the internal region of the protein sequence according to NP_542937.1, NP_542938.1, NP_004383.2.
Specificity This antibody is expected to recognise all three reported isoforms(NP_542937.1, NP_542938.1, NP_004383.2).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Dachshund homolog 1
Background Synonyms: OTTHUMP00000040839, dachshund homolog 1 (Drosophila), DACH1, RP11-512J14.1, DACH, FLJ10138, dachshund homolog 1
Gene ID 1602, 13134
NCBI Accession NP_542938, NP_542937, NP_004383
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75 and 52 kDa bands observed in Human Kidney lysates (calculated MW of 73.0 kDa according to NP_542937.1 and of 51.9 kDa according to NP_004383.2). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Dachshund Homolog 1 (Drosophila) (DACH1) (Internal Region) antibody ABIN334382 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Wu, Yang, Wang et al.: "DACH1 inhibits transforming growth factor-beta signaling through binding Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51673-84, 2003 (PubMed).

Validation Images
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