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Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E) (Internal Region) antibody

Antigen

Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E)
Synonyms SemD, SEMA1, SEMAD, SEMAL, coll-1, Hsema-I, SEMAIII, Hsema-III, MGC133243, SEMA3E, fj37c11, wu:fj37c11, si:ch211-120m13.2
Binding Site
Alternatives

Internal Region
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
1 reference available
Catalog no. ABIN334487
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Alternative name Semaphorin 3E
Gene ID 9723, 20349 (mouse)
Immunogen C-NGGRYGTTKDYPDD
Description All names and symbols: SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5 , sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E. Official Symbol: SEMA3E. RefSeq number(s): NP_036563.1
Specificity Human, Mouse, Dog

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Gu, Yoshida, Livet et al.: "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins." in: Science (New York, N.Y.), Vol. 307, Issue 5707, pp. 265-8, 2005 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E)", type "Antibodies"
Hosts Rabbit (25), Goat (8), Mouse (1)
Reactivities Human (29), Mouse (Murine) (22), Cow (Bovine) (18), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18), Rat (Rattus) (18), Sheep (Ovine) (18), Dog (Canine) (1)
Applications ELISA (15), Immunofluorescence (IF) (15), Western Blotting (WB) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes AA 401-450 (18), C-Term (5), Internal Region (2)