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Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody

Details for Product No. ABIN334493, Supplier: Log in to see
Antigen
  • FLFL1
  • KIAA2010
  • PP4R3A
  • smk-1
  • smk1
  • 1110034C04Rik
  • BC064465
  • mKIAA2010
  • RGD1309059
Epitope
Internal Region
5
3
1
1
Reactivity
Human
18
6
5
2
2
1
1
1
1
1
Host
Goat
11
7
Clonality
Polyclonal
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
13
5
3
2
2
Supplier
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Immunogen Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.
Blocking Peptide Blocking peptide for this product available: ABIN369053
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMEK1 / KIAA2010 (SMEK1 Antibody Abstract)
Background Synonyms: SMEK homolog 1, suppressor of mek1 (Dictyostelium), KIAA2010, FLFL1, MSTP033, smk-1, smk1, SMEK homolog 1, suppressor of mek1
Gene ID 55671, 68734, 314388
NCBI Accession NP_115949
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110 kDa band observed in Human Pancreas lysates (calculated MW of 93.9 kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody (ABIN334493) ABIN334493 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer)...
Background publications Panowski, Wolff, Aguilaniu et al.: "PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans." in: Nature, Vol. 447, Issue 7144, pp. 550-5, 2007 (PubMed).