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Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody

Details for Product No. ABIN334493
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Antigen
Synonyms FLFL1, KIAA2010, PP4R3A, smk-1, smk1, 1110034C04Rik, BC064465, mKIAA2010, RGD1309059
Epitope
Internal Region
(3), (3), (1), (1)
Reactivity
Human
(9), (2), (1), (1), (1)
Host
Goat
(7), (4)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(8), (3), (3), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN334493
374.27 $
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Immunogen Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMEK1 / KIAA2010
Background Synonyms: SMEK homolog 1, suppressor of mek1 (Dictyostelium), KIAA2010, FLFL1, MSTP033, smk-1, smk1, SMEK homolog 1, suppressor of mek1
Gene ID 55671, 68734, 314388
NCBI Accession NP_115949.3
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110 kDa band observed in Human Pancreas lysates (calculated MW of 93.9 kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody ABIN334493 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Panowski, Wolff, Aguilaniu et al.: "PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans." in: Nature, Vol. 447, Issue 7144, pp. 550-5, 2007 (PubMed).

Validation Images
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