Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody

Details for Product No. ABIN334493
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Synonyms FLFL1, KIAA2010, PP4R3A, smk-1, smk1, BC064465, mKIAA2010, 1110034C04Rik, RGD1309059
Internal Region
(3), (1), (1)
(5), (2), (1), (1), (1)
(4), (4)
Methode Type Peptide ELISA
ELISA, Western Blotting (WB)
(7), (3), (1)
Pubmed 1 reference available
Catalog no. ABIN334493
Quantity 100 µg
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Immunogen Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMEK1 / KIAA2010
Background Synonyms: SMEK homolog 1, suppressor of mek1 (Dictyostelium), KIAA2010, FLFL1, MSTP033, smk-1, smk1, SMEK homolog 1, suppressor of mek1
Gene ID 55671, 68734, 314388
NCBI Accession NP_115949.3
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 110 kDa band observed in Human Pancreas lysates (calculated MW of 93.9 kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1) (Internal Region) antibody ABIN334493 (0.3µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Panowski, Wolff, Aguilaniu et al.: "PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans." in: Nature, Vol. 447, Issue 7144, pp. 550-5, 2007 (PubMed).

Hosts (4), (4)
Reactivities (5), (2), (1), (1), (1)
Applications (7), (3), (1)
Epitopes (3), (1), (1)
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