CD175s (Sialosyl-Tn Antigen) antibody (FITC)

Catalog No. ABIN336911

Product Details

Target: CD175s (Sialosyl-Tn Antigen)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Specificity: NeuAc alpha GalNacOSer/Thr. Molecular Weight: several HM weight glycoproteins.

Immunogen: Ovine submaxillary mucin (OSM)

Clone: STn218

Isotype: IgG1 kappa

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Human gastrointestinal tumor, prostate, ovary ca. Cellular Localization: Cytoplasmic, apical membrane, luminal.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibodies are supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description Sialosyl Tn antigen also known as CD175s is expressed in the major

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: CD175s (Sialosyl-Tn Antigen)