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Autophagy Related 10 (ATG10) antibody

Details for Product No. ABIN350090, Supplier: Log in to see
Antigen
  • APG10
  • APG10L
  • pp12616
  • 5330424L23Rik
  • 5430428K15Rik
  • AI852123
  • Agp10
  • Apg10l
  • Apg10p
  • Atg10l
Reactivity
Human
68
26
26
1
1
Host
Sheep
65
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
50
28
25
11
6
2
1
Supplier
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Immunogen A synthetic peptide from human ATG10 conjugated to an immunogenic carrier protein has been used as the antigen.
Isotype IgG
Specificity Specific for ATG10.
Alternative Name ATG10 (ATG10 Antibody Abstract)
Background Autophagocytosis is a starvation-induced process responsible for transport of cytoplasmic proteins to the vacuole.
Function: Plays a role inautophagy. ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule.
Subunit: Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7.
Subcellular location: Cytoplasm. Also known as: PP12616, APG10L, APG10-like, APG10, APG10L, pp12616, FLJ13954, DKFZP586I0418, ATG10autophagy related 10 homolog (S. cerevisiae).
Pathways
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Wan, Gong, Qin et al.: "Large-scale cDNA transfection screening for genes related to cancer development and progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 44, pp. 15724-9, 2004 (PubMed).

Wiemann, Arlt, Huber et al.: "From ORFeome to biology: a functional genomics pipeline." in: Genome research, Vol. 14, Issue 10B, pp. 2136-44, 2004 (PubMed).

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).