T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) antibody

Antigen: T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1)

Synonyms: FLJ36302, AI847750, D16Ium10, D16Ium10e, TIAM1, MGC142564

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN351008

Additional Information

Alternative name: T-lymphoma invasion and metastasis-inducing protein 1 (TIAM-1, TIAM1)

UniProt: Q13009

Immunogen: A synthetic peptide as a part of human, mouse T-lymphoma invasion and metastasis-inducing protein 1 (TIAM-1, TIAM1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates RAC1, CDC42, and to a lesser extent RHOA. SUBUNIT: Interacts with BAIAP2. TISSUE SPECIFICITY: Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas. Also known as: TIAM-1, TIAM1.

Specificity: Appears to be specific for TIAM1.

Application Details

Application Notes: IHC. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Habets, van der Kammen, Stam et al.: "Sequence of the human invasion-inducing TIAM1 gene, its conservation in evolution and its expression in tumor cell lines of different tissue origin." in: Oncogene, Vol. 10, Issue 7, pp. 1371-6, 1995 (PubMed).

Habets, van der Kammen, Jenkins et al.: "The invasion-inducing TIAM1 gene maps to human chromosome band 21q22 and mouse chromosome 16." in: Cytogenetics and cell genetics, Vol. 70, Issue 1-2, pp. 48-51, 1995 (PubMed).

Michiels, Habets, Stam et al.: "A role for Rac in Tiam1-induced membrane ruffling and invasion." in: Nature, Vol. 375, Issue 6529, pp. 338-40, 1995 (PubMed).

Hattori, Fujiyama, Taylor et al.: "The DNA sequence of human chromosome 21." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000 (PubMed).

Worthylake, Rossman, Sondek: "Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1." in: Nature, Vol. 408, Issue 6813, pp. 682-8, 2000 (PubMed).

Connolly, Rice, Feig et al.: "Tiam1-IRSp53 complex formation directs specificity of rac-mediated actin cytoskeleton regulation." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4602-14, 2005 (PubMed).

Sjöblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Cao, Yu, Banh et al.: "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5864-76, 2007 (PubMed).