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Pan Cytokeratin antibody

Details for Product No. ABIN356306
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Antigen
Reactivity
Human
(21), (10), (5), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1)
Host
Mouse
(19), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(21), (15), (11), (11), (9), (5), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 1.2 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN356306
533.50 $
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Immunogen Human epidermal keratin. Remarks: Molecular weight of antigen: CK1 (67), CK2 (65. 5), CK3 (64), CK4 (59) CK5 (58),CK6 (56), CK8 (52. 5), CK10 (56. 5), CK13 (54) CK14 (50), CK15 (50), CK16 (48), CK18 (45), CK 19(40).
Clone Cocktail
Isotype Cocktail
Characteristics Synonyms: pan Keratin, Cytokeratin Pan-reactive
Purification Purified
Background Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeletonof epithelial tissue There are two types of Cytokeratins: the low weight, acidic type Icytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found inpairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weightcytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67),CK2(65. 5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52. 5) and CK9. The lowmolecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10(56. 5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20(46).
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Immunofluorescence. Recommended Positive Control: Human skin, lung, colon, prostate, or any GI tissue orcancer tissue's. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Sun, Tseng, Huang et al.: "Monoclonal antibody studies of mammalian epithelial keratins: a review." in: Annals of the New York Academy of Sciences, Vol. 455, pp. 307-29, 1986 (PubMed).

Tseng, Jarvinen, Nelson et al.: "Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies." in: Cell, Vol. 30, Issue 2, pp. 361-72, 1983 (PubMed).

Validation Images
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