Cytokeratin 1 antibody (AA 141-240)

Catalog No. ABIN733343

Product Details anti-Cytokeratin 1 Antibody

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Binding Specificity: AA 141-240

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Cow, Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human pan cytokeratin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cytokeratin 1 antibody (ABIN733343)

Yang, Li, Qi, Li, Zhou, Qing, Zhang, Gao: "lncRNA H19 is involved in TGF-β1-induced epithelial to mesenchymal transition in bovine epithelial cells through PI3K/AKT Signaling Pathway." in: PeerJ, Vol. 5, pp. e3950, (2017) (PubMed).

Wang, Jin, Fan, Wang, Xing, Niu: "Anti-proliferative activities of finasteride in benign prostate epithelial cells require stromal fibroblasts and c-Jun gene." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172233, (2017) (PubMed).

Chen, Zheng, Liu, Shi, Qi: "Periostin activates pathways involved in epithelial-mesenchymal transition in adamantinomatous craniopharyngioma." in: Journal of the neurological sciences, Vol. 360, pp. 49-54, (2016) (PubMed).

Li, Wang, Liu, Tu, He, Wang, Han, Li, Li, Tao, Cai: "Identification of apoptotic cells in the thymus of piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus." in: Virus research, Vol. 189, pp. 29-33, (2014) (PubMed).

Li, Jia, Jia, Shi, Li, Ye, Wang, Xiong, Wang, Li: "Enrichment of Oct3/4-positive cells from a human bronchial epithelial cell line." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 121, Issue 7, pp. 612-25, (2013) (PubMed).

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin (KRT1 Products)

Synonyms: CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, Krt-2.1 antibody, Krt2-1 antibody, Krt86 antibody, KRT1 antibody, Kb1 antibody, KER1 antibody, CK-1 antibody, Keratin-1 antibody, keratin 1 antibody, KRT1 antibody, Krt1 antibody

Background:

Synonyms: pan-cytokeratin, pan-CK, pan CK, P-CK, wide spectrum Cytokeratin, Cytokeratins, [cytokeratins 1, 2,4,5,6,7,8,71,72,75,78].

Background: Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.