SMT3 Suppressor of Mif Two 3 Homolog 4 (S. Cerevisiae) (SUMO4) (Wild Type) antibody

Details for Product No. ABIN356786
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Antigen
Synonyms IDDM5, SMT3H4, SUMO-4, dJ281H8.4
Epitope
Wild Type
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(37)
Host
Rabbit
(34), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(37), (25), (20), (8), (2), (2)
Pubmed 7 references available
Quantity 0.4 mL
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Catalog No. ABIN356786
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Immunogen This antibody is generated from rabbits immunized with the KLH conjugated peptide CEPRGLS(M)KQIRFRFG selected from human SUMO4. This antibody is peptide affinity purified using peptides CEPRGLS(M)KQIRFRFG (positive selection) and CEPRGLS(V)KQIRFRFG (negative selection).
Specificity This antibody is specific to SUMO4 (M55 Wild type).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name SUMO4 / SMT3H4
Background SUMO4 is a member of the SUMO gene family. This family of small ubiquitin-related modifiers covalently modify target lysines in proteins and control the target proteins' subcellular localization, stability, or activity. Upon oxidative stress, SUMO4 conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. This protein may also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to SUMO4 substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. In contrast to SUMO1, SUMO2 and SUMO3, SUMO4 seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. SUMO4 is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B- dependent transcription of the IL12B gene. The M55V substitution has been associated with type I diabetes.
Alternate names: SUMO-4, Small ubiquitin-like protein 4, Small ubiquitin-related modifier 4
Gene ID 387082, 9606
UniProt Q6EEV6
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-SMT3 Suppressor of Mif Two 3 Homolog 4 (S. Cerevisiae) (SUMO4) (Wild Type) antibody anti-SMT3 Suppressor of Mif Two 3 Homolog 4 (S. Cerevisiae) (SUMO4) (Wild Type) antibody
anti-SMT3 Suppressor of Mif Two 3 Homolog 4 (S. Cerevisiae) (SUMO4) (Wild Type) antibody (2) anti-SMT3 Suppressor of Mif Two 3 Homolog 4 (S. Cerevisiae) (SUMO4) (Wild Type) antibody (Image 2)
Background publications Ohshima, Shimotohno: "Transforming growth factor-beta-mediated signaling via the p38 MAP kinase pathway activates Smad-dependent transcription through SUMO-1 modification of Smad4." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 50833-42, 2003 (PubMed).

Bailey, OHare: "Characterization of the localization and proteolytic activity of the SUMO-specific protease, SENP1." in: The Journal of biological chemistry, Vol. 279, Issue 1, pp. 692-703, 2003 (PubMed).

Pountney, Huang, Burns et al.: "SUMO-1 marks the nuclear inclusions in familial neuronal intranuclear inclusion disease." in: Experimental neurology, Vol. 184, Issue 1, pp. 436-46, 2003 (PubMed).

Ling, Sankpal, Robertson et al.: "Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription." in: Nucleic acids research, Vol. 32, Issue 2, pp. 598-610, 2004 (PubMed).

Yang, Sharrocks: "SUMO promotes HDAC-mediated transcriptional repression." in: Molecular cell, Vol. 13, Issue 4, pp. 611-7, 2004 (PubMed).

Bohren, Nadkarni, Song et al.: "A M55V polymorphism in a novel SUMO gene (SUMO-4) differentially activates heat shock transcription factors and is associated with susceptibility to type I diabetes mellitus." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27233-8, 2004 (PubMed).

Guo, Li, Zhang et al.: "A functional variant of SUMO4, a new I kappa B alpha modifier, is associated with type 1 diabetes." in: Nature genetics, Vol. 36, Issue 8, pp. 837-41, 2004 (PubMed).

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