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Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody

Details for Product No. ABIN357745, Supplier: Log in to see
Antigen
  • EZH1
  • KMT6B
  • AW557915
  • mKIAA0388
  • si:ch211-250i3.1
  • zgc:136829
  • enx-1
  • enx1
  • ezh1
  • ezh2
  • kmt6
  • xez
Epitope
Middle Region
26
18
11
3
2
1
1
1
Reactivity
Human
91
47
35
7
3
2
1
1
1
1
1
1
Host
Rabbit
78
15
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
75
48
34
13
11
9
3
1
1
1
1
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EZH1.
Isotype Ig
Specificity This antibody is specific to EZH1 (Center).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EZH1 (EZH1 Antibody Abstract)
Background EZH1 encodes a protein of 747 amino acids that displays 55 % amino acid identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated.. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene.Synonyms: ENX-2, Enhancer of zeste homolog 1, Histone-lysine N-methyltransferase EZH1, KIAA0388
Molecular Weight 85270 Da
Gene ID 2145, 9606
UniProt Q92800
Research Area Chromatin and Nuclear Signaling
Pathways
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody (ABIN357745) anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody
 image for anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody (ABIN357745) anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody (Image 2)
Background publications Ogawa, Hiraoka, Taniguchi et al.: "Cloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2." in: Biochimica et biophysica acta, Vol. 1395, Issue 2, pp. 151-8, 1998 (PubMed).

Abel, Brody, Valdes et al.: "Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1." in: Genomics, Vol. 37, Issue 2, pp. 161-71, 1997 (PubMed).

Friedman, Ostermeyer, Lynch et al.: "22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors." in: Genomics, Vol. 25, Issue 1, pp. 256-63, 1995 (PubMed).

Osborne-Lawrence, Welcsh, Spillman et al.: "Direct selection of expressed sequences within a 1-Mb region flanking BRCA1 on human chromosome 17q21." in: Genomics, Vol. 25, Issue 1, pp. 248-55, 1995 (PubMed).

Brody, Abel, Castilla et al.: "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17." in: Genomics, Vol. 25, Issue 1, pp. 238-47, 1995 (PubMed).