Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody

Details for Product No. ABIN357745
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Antigen
Synonyms EZH1, KMT6B, AW557915, mKIAA0388, si:ch211-250i3.1, zgc:136829, enx-1, enx1, ezh1, ezh2, kmt6, xez
Epitope
Middle Region
(17), (10), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(63), (37), (25), (16), (14), (13), (13), (12), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(56), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(53), (30), (20), (10), (9), (4), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN357745
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human EZH1.
Isotype Ig
Specificity This antibody is specific to EZH1 (Center).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name EZH1
Background EZH1 encodes a protein of 747 amino acids that displays 55 % amino acid identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated.. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene.Synonyms: ENX-2, Enhancer of zeste homolog 1, Histone-lysine N-methyltransferase EZH1, KIAA0388
Molecular Weight 85270 Da
Gene ID 2145, 9606
UniProt Q92800
Research Area Chromatin and Nuclear Signaling
Application Notes ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody
anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody (2) anti-Enhancer of Zeste Homolog 1 (Drosophila) (EZH1) (Middle Region) antibody (Image 2)
Background publications Brody, Abel, Castilla et al.: "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17." in: Genomics, Vol. 25, Issue 1, pp. 238-47, 1995 (PubMed).

Osborne-Lawrence, Welcsh, Spillman et al.: "Direct selection of expressed sequences within a 1-Mb region flanking BRCA1 on human chromosome 17q21." in: Genomics, Vol. 25, Issue 1, pp. 248-55, 1995 (PubMed).

Friedman, Ostermeyer, Lynch et al.: "22 genes from chromosome 17q21: cloning, sequencing, and characterization of mutations in breast cancer families and tumors." in: Genomics, Vol. 25, Issue 1, pp. 256-63, 1995 (PubMed).

Abel, Brody, Valdes et al.: "Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1." in: Genomics, Vol. 37, Issue 2, pp. 161-71, 1997 (PubMed).

Ogawa, Hiraoka, Taniguchi et al.: "Cloning and expression of a human/mouse Polycomb group gene, ENX-2/Enx-2." in: Biochimica et biophysica acta, Vol. 1395, Issue 2, pp. 151-8, 1998 (PubMed).

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