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serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) (C-Term) antibody

Details for Product No. ABIN357774
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Antigen
Synonyms Dcpp-2, EG630537, SPT-2, LCB2, LCB2a, SPTLC2, HSN1C, LCB2A, NSAN1C, SPT2, hLCB2a, AI173915, mKIAA0526, Pomt2, sptlc2, zgc:110661
Epitope
C-Term
(14), (8), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(53), (31), (27), (19), (18), (18), (2), (1), (1), (1)
Host
Rabbit
(53), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(40), (24), (14), (8), (2), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN357774
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPTLC2.
Specificity This antibody is specific to SPTLC2 (C-term).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name SPTLC2
Background Serine palmitoyltransferase (SPT) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine.
Alternate names: KIAA0526, LCB 2, LCB2, LCB2a, Long chain base biosynthesis protein 2, SPT 2, SPT2, Serine palmitoyltransferase 2, Serine-palmitoyl-CoA transferase 2
Molecular Weight 62924 Da
Gene ID 9517
NCBI Accession 5874
UniProt O15270
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) (C-Term) antibody anti-serine Palmitoyltransferase, Long Chain Base Subunit 2 (SPTLC2) (C-Term) antibody
Background publications Takeda, Yano, Eng et al.: "A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones." in: Human molecular genetics, Vol. 2, Issue 11, pp. 1793-8, 1994 (PubMed).

Hillier, Lennon, Becker et al.: "Generation and analysis of 280,000 human expressed sequence tags." in: Genome research, Vol. 6, Issue 9, pp. 807-28, 1997 (PubMed).

Weiss, Stoffel: "Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis." in: European journal of biochemistry / FEBS, Vol. 249, Issue 1, pp. 239-47, 1997 (PubMed).

Dias Neto, Correa, Verjovski-Almeida et al.: "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 7, pp. 3491-6, 2000 (PubMed).

Stachowitz, Alessandrini, Abeck et al.: "Permeability barrier disruption increases the level of serine palmitoyltransferase in human epidermis." in: The Journal of investigative dermatology, Vol. 119, Issue 5, pp. 1048-52, 2002 (PubMed).

Validation Images
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