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Details for Product No. ABIN360515

Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term) antibody

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BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1, AU045529, bub1b, BUB1, BUB1-like, BubR1, BubRI, CG14030, CG14030/Bub1, Dmel\\CG14030, bub1, 31/13, BcDNA:LD23835, Bub1, CG 7838, CG7838, DmBUB1, Dmbu ... show more
»Alternatives N-Term
»Alternatives Human
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN360515
Quantity 0.4 mL
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BUB1B.
Specificity This antibody reacts to BUBR1 (BUB1B).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name BUB1B
Background BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.
Alternate names: BUB1 beta, BUBR1, MAD3/BUB1-related protein kinase, MAD3L, Mitotic checkpoint kinase MAD3L, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Protein SSK1
Gene ID 701
NCBI Accession 9606
UniProt O60566
Research Area Cell Cycle
Application Notes ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Cahill, Lengauer, Yu et al.: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, 1998 (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).

Chan, Schaar, Yen: "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 49-63, 1998 (PubMed).

Davenport, Fernandes, Harris et al.: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, 1999 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

Alternatives for antigen "Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B)", type "Antibodies"
Hosts (53), (40), (9)
Reactivities (93), (43), (12), (5), (3), (1)
Applications (88), (42), (25), (23), (19), (14), (10), (8), (7), (7), (2), (2), (1), (1)
Conjugates (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (9), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)