Podoplanin (PDPN) antibody

Details for Product No. ABIN360956
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Antigen
Synonyms PDPN, AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, OTS-8, RANDAM-2, T1-alpha, T1a, T1alpha, E11, RTI40
Reactivity
Rat (Rattus)
(122), (48), (20), (1)
Host
Mouse
(82), (53), (15), (13), (4), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(107), (48), (40), (37), (31), (28), (20), (19), (12), (10), (5), (4), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN360956
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Immunogen BALB/c mice using a membrane protein fraction of isolated Rat glomeruli
Clone LF3(B7)D5B3
Specificity This antibody recognizes Rat Podoplanin.
Purification Protein G Chromatography
Alternative Name Podoplanin
Background Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor. It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Gene ID 54320
NCBI Accession NP_062231
UniProt Q64294
Application Notes Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 6.0 without preservatives and stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for two weeks or (in aliquots) at -20 °C for longer.
Background publications Breiteneder-Geleff, Matsui, Soleiman et al.: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).

Schacht, Ramirez, Hong et al.: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

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