Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) (pSer845) antibody

Details for Product No. ABIN361477
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Antigen
Synonyms GRIA1, glur1, GluA1, gluR-A, GLUH1, GLUR1, GLURA, HBGR1, 2900051M01Rik, Glr-1, Glr1, GluR-A, GluRA, Glur-1, Glur1, HIPA1, gluR-K1
Epitope
pSer845
(44), (42), (35), (26), (23), (22), (13), (13), (12), (12), (6), (6), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(233), (205), (172), (6), (5), (4), (2), (2), (1)
Host
Rabbit
(244), (48), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(223), (85), (57), (50), (48), (40), (28), (9), (8), (3), (2), (1)
Pubmed 27 references available
Quantity 150 μL
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.
Specificity The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with λ-Ptase.
Purification Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name GluR1 (GRIA1 Antibody Abstract)
Background Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as arning and memory (Lee at al., 2003; Esteban at al., 2003).
Pathways PI3K-Akt Signaling Pathway
Application Notes WB: 1:1000. IHC: 1:1000 (frozen sections).
Restrictions For Research Use only
Format Liquid
Buffer 100 myl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 myg per ml BSA and 50% glycerol.
Storage -20 °C
Supplier Images
anti-Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) (pSer845) antibody Western blots of rat hippocampal lysate showing specific immunolabeling of the ~100k ...
Product cited in: Tassin, Benavides, Plattner et al.: "Regulation of ERK Kinase by MEK1 Kinase Inhibition in the Brain." in: The Journal of biological chemistry, Vol. 290, Issue 26, pp. 16319-29, 2015 (PubMed).

Mao, Xue, Jin et al.: "Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine." in: Journal of neurochemistry, Vol. 133, Issue 6, pp. 795-805, 2015 (PubMed).

Ip, Fu, Cheng et al.: "Anemoside A3 Enhances Cognition through the Regulation of Synaptic Function and Neuroprotection." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 40, Issue 8, pp. 1877-87, 2015 (PubMed).

Suzuki, Harada, Shiraishi et al.: "In vivo pharmacological characterization of TAK-063, a potent and selective phosphodiesterase 10A inhibitor with antipsychotic-like activity in rodents." in: The Journal of pharmacology and experimental therapeutics, Vol. 352, Issue 3, pp. 471-9, 2015 (PubMed).

Wang, Zhang, Xu et al.: "Fluoxetine improves behavioral performance by suppressing the production of soluble β-amyloid in APP/PS1 mice." in: Current Alzheimer research, Vol. 11, Issue 7, pp. 672-80, 2014 (PubMed).

Mao, Hastings, Fibuch et al.: "Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice." in: European journal of pharmacology, Vol. 738, pp. 237-44, 2014 (PubMed).

Bernard, Castano, Bayer et al.: "Necessary, but not sufficient: insights into the mechanisms of mGluR mediated long-term depression from a rat model of early life seizures." in: Neuropharmacology, Vol. 84, pp. 1-12, 2014 (PubMed).

Xue, Edwards, Mao et al.: "Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration." in: Neurochemistry international, Vol. 64, pp. 48-54, 2013 (PubMed).

Shen, Fu, Cheng et al.: "Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 464-72, 2013 (PubMed).

Davis, Olausson, Greengard et al.: "Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits." in: The European journal of neuroscience, Vol. 35, Issue 2, pp. 300-8, 2012 (PubMed).

Torres-Altoro, Mathur, Drerup et al.: "Organophosphates dysregulate dopamine signaling, glutamatergic neurotransmission, and induce neuronal injury markers in striatum." in: Journal of neurochemistry, Vol. 119, Issue 2, pp. 303-13, 2011 (PubMed).

Kuroiwa, Snyder, Shuto et al.: "Phosphodiesterase 4 inhibition enhances the dopamine D1 receptor/PKA/DARPP-32 signaling cascade in frontal cortex." in: Psychopharmacology, 2011 (PubMed).

Rebholz, Nishi, Liebscher et al.: "CK2 negatively regulates Galphas signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14096-101, 2009 (PubMed).

Santini, Heiman, Greengard et al.: "Inhibition of mTOR signaling in Parkinson's disease prevents L-DOPA-induced dyskinesia." in: Science signaling, Vol. 2, Issue 80, pp. ra36, 2009 (PubMed).

Zheng, Keifer: "PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning." in: Journal of neurophysiology, Vol. 101, Issue 5, pp. 2539-49, 2009 (PubMed).

Nishi, Kuroiwa, Miller et al.: "Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 42, pp. 10460-71, 2008 (PubMed).

Background publications Sun, Milovanovic, Zhao et al.: "Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 16, pp. 4216-30, 2008 (PubMed).

Lee, Takamiya, Han et al.: "Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory." in: Cell, Vol. 112, Issue 5, pp. 631-43, 2003 (PubMed).

Esteban, Shi, Wilson et al.: "PKA phosphorylation of AMPA receptor subunits controls synaptic trafficking underlying plasticity." in: Nature neuroscience, Vol. 6, Issue 2, pp. 136-43, 2003 (PubMed).

Banke, Bowie, Lee et al.: "Control of GluR1 AMPA receptor function by cAMP-dependent protein kinase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 1, pp. 89-102, 2000 (PubMed).

Roche, OBrien, Mammen et al.: "Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit." in: Neuron, Vol. 16, Issue 6, pp. 1179-88, 1996 (PubMed).

Hollmann, Heinemann: "Cloned glutamate receptors." in: Annual review of neuroscience, Vol. 17, pp. 31-108, 1994 (PubMed).

Keinänen, Wisden, Sommer et al.: "A family of AMPA-selective glutamate receptors." in: Science (New York, N.Y.), Vol. 249, Issue 4968, pp. 556-60, 1990 (PubMed).

General Ferrario, Loweth, Milovanovic et al.: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, Vol. 490, Issue 3, pp. 180-4, 2011 (PubMed).

Santini, Sgambato-Faure, Li et al.: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, 2010 (PubMed).

Napolitano, Bonito-Oliva, Federici et al.: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, 2010 (PubMed).

Sears, Liu, Narayanan et al.: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010 (PubMed).

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Catalog No. ABIN361477
401.50 $
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Quantity
Price
150 μL
401.50 $

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