Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) (pSer845) antibody

Antigen: Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)

Synonyms: GLUH1, GLUR1, GLURA, HBGR1, MGC133252, Glr1, Glr-1, GluA1, GluRA, Glur1, HIPA1, GluR-A, Glur-1, AI853806, 2900051M01Rik, gluR-A, GRIA1, gria1

Binding Site: pSer845

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361477

Additional Information

Alternative name: GluR1

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.

Description: Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as arning and memory (Lee at al., 2003; Esteban at al., 2003).

Specificity: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with λ-Ptase.

Application Details

Application Notes: WB: 1:1000. IHC: 1:1000 (frozen sections).

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Buffer: 100 myl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 myg per ml BSA and 50% glycerol.

Storage: For long term storage –20°C is recommended. Stable at –20°C for at least 1 year.

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Publications

Sears, Liu, Narayanan et al.: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010 (PubMed).

Napolitano, Bonito-Oliva, Federici et al.: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, 2010 (PubMed).

Santini, Sgambato-Faure, Li et al.: "Distinct changes in cAMP and extracellular signal-regulated protein kinase signalling in L-DOPA-induced dyskinesia." in: PLoS ONE, Vol. 5, Issue 8, pp. e12322, 2010 (PubMed).

Ferrario, Loweth, Milovanovic et al.: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, 2011 (PubMed).

Product: Kuroiwa, Snyder, Shuto et al.: "Phosphodiesterase 4 inhibition enhances the dopamine D1 receptor/PKA/DARPP-32 signaling cascade in frontal cortex." in: Psychopharmacology, 2011 (PubMed).

Torres-Altoro, Mathur, Drerup et al.: "Organophosphates dysregulate dopamine signaling, glutamatergic neurotransmission, and induce neuronal injury markers in striatum." in: Journal of neurochemistry, Vol. 119, Issue 2, pp. 303-13, 2011 (PubMed).

Davis, Olausson, Greengard et al.: "Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits." in: The European journal of neuroscience, Vol. 35, Issue 2, pp. 300-8, 2012 (PubMed).