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CAMK2G antibody (Calcium/calmodulin-Dependent Protein Kinase II gamma)

Details for Product anti-CAMK2G Antibody No. ABIN361698, Supplier: Login to see New
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Human, Mouse (Murine), Rat (Rattus)
(123), (80), (77), (21), (4), (3), (3), (2), (2), (2), (1), (1), (1)
(93), (46)
Clonality (Clone)
Monoclonal ()
This CAMK2G antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(129), (69), (66), (58), (47), (41), (19), (10), (4), (3), (2), (1), (1), (1)
Pubmed 14 references available
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Quantity 25 μg
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Synthetic peptide
Clone 22B1
Specificity Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Sensitivity 1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Purification Protein G Purified
Alternative Name CaMKII (CAMK2G Antibody Abstract)
Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Cellular Localization: Cytoplasm | Mitochondrion | Nucleus | Cell Junction | Synapse | Presynaptic Cell Membrane
Gene ID 25400
NCBI Accession NP_037052
UniProt P11275
Research Area Cancer, Hypertrophy, Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway
Application Notes Recommended Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:1000), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-CAMK2G antibody (Calcium/calmodulin-Dependent Protein Kinase II gamma) (ABIN361698) CamKII (22B1), Mice ventricles.
Product cited in: Feng, Szabo, Dziak et al.: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010 (PubMed).

Background publications Shifman, Choi, Mihalas et al.: "Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 38, pp. 13968-73, 2006 (PubMed).

Hughes, Edin, Antonsson et al.: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001 (PubMed).

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000 (PubMed).

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999 (PubMed).

Makhinson, Chotiner, Watson et al.: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999 (PubMed).

Leonard, Lim, Hemsworth et al.: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999 (PubMed).

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998 (PubMed).

Barria, Muller, Derkach et al.: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997 (PubMed).

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997 (PubMed).

Nghiem, Saati, Martens et al.: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993 (PubMed).

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994 (PubMed).

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995 (PubMed).

Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987 (PubMed).

Catalog No. ABIN361698
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