Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody

Details for Product No. ABIN361698
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Antigen
Synonyms cam2, camk2, camk2g, camkII, camkb, CaMKII, R74975, mKIAA0968, CAMK, CAMK-II, CAMKG, 5930429P18Rik, Camkg
Reactivity
Rat (Rattus)
(65), (46), (37), (14), (5), (3), (2), (2), (1), (1), (1), (1)
Host
Mouse
(64), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(63), (20), (17), (17), (10), (8), (7), (6), (5), (4), (3), (1)
Pubmed 13 references available
Quantity 25 µg
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Catalog No. ABIN361698
190.30 $
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Immunogen Synthetic peptide
Clone 22B1
Isotype IgG1
Specificity Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Sensitivity 1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Purification Protein G Purified
Alternative Name CaMKII
Background Synonyms:
CamK2, CamK2A, CamK2B, CamK2D, CamK2G, CAMKA
CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa(beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site. The binding of Ca2+auto inhibitory effect and activates the kinase. /calmodulin to its regulatory domain releases itsThis kinase activation results in auto phosphorylation at threonine 286.The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Auto phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities.
Gene ID 25400
NCBI Accession NP_037052
UniProt P11275
Research Area Cancer, Hypertrophy, Kinases/Phosphatases, Cell Signaling
Application Notes Recommended Dilution: 1 µ/mL was sufficient for detection of 0.2 µg CAMKII by colorimetric immunoblot
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody CamKII (22B1), Mice ventricles.
Background publications Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987 (PubMed).

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995 (PubMed).

Nghiem, Saati, Martens et al.: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993 (PubMed).

Barria, Muller, Derkach et al.: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997 (PubMed).

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998 (PubMed).

Leonard, Lim, Hemsworth et al.: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999 (PubMed).

Makhinson, Chotiner, Watson et al.: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999 (PubMed).

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999 (PubMed).

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000 (PubMed).

Hughes, Edin, Antonsson et al.: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001 (PubMed).

Shifman, Choi, Mihalas et al.: "Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by calmodulin with two bound calciums." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 38, pp. 13968-73, 2006 (PubMed).

General Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994 (PubMed).

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997 (PubMed).

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