HLA-DR-gamma (CD74) antibody

Antigen: HLA-DR-gamma (CD74)

Synonyms: DHLAG, HLADG, Ia-GAMMA, Ii, CLIP

Clonality: Monoclonal (PIN-1)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Flow Cytometry (FACS)

Catalog no.: ABIN361789

Additional Information

Alternative name: Cd74

Immunogen: Human CD74 invariant chain sythetic peptide

Format: Ig-fraction

Isotype: IgG  (Matching secondary antibodies)

Clone: PIN-1

Description: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Specificity: Detects ~33-35 kDa protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots. Species cross-reactivity: Human.

Application Details

Application Notes: 1 ug/mL was sufficient for detection using colorimetric western blot analysis

Concentration: 1.0mg/mL

Buffer: Mouse immunoglobulin in PBS, pH 7.2, 0.09% azide and 50% glycerol

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

CD-74 (PIN 1.1) N87 lysates mixed with Macrophage inhibitory factor