CD28 antibody (Biotin)

Catalog No. ABIN371357

Product Details anti-CD28 Antibody (Biotin)

Target: CD28

Reactivity: Chicken, Turkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50 % amino acid sequence identity with mammalian CD28.

Characteristics: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Clone: AV7

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Background: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma/delta+ T cells are CD28-negative. (Ref.1) Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both Vbeta1- and Vbeta2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3. (Ref.2)Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 396249

UniProt: P31043

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response