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Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody

Details for Product No. ABIN371649, Supplier: Log in to see
Antigen
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
Alternatives
anti-Human Second Mitochondria-Derived Activator of Caspase antibody for Western Blotting
Reactivity
Human
134
55
35
4
4
3
2
1
1
Host
Mouse
115
28
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
139
57
56
32
21
10
8
6
4
3
2
2
2
1
1
1
1
1
Supplier
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Clone 2D12
Isotype IgG2b
Specificity This antibody is specific for Human SMAC/DIABLO.
Characteristics Synonyms: Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, DirectIAP-binding protein with low pI
Purification Purified
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background SMAC/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. SMAC/DIABLO has been shown to neutralize the antiapoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis. (Ref.1-6)Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA: 1/1,000-1/2,000. Western Blot: 1/5,000-1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Hu, Yang: "Cellular inhibitor of apoptosis 1 and 2 are ubiquitin ligases for the apoptosis inducer Smac/DIABLO." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10055-60, 2003 (PubMed).

Adrain, Creagh, Martin: "Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2." in: The EMBO journal, Vol. 20, Issue 23, pp. 6627-36, 2001 (PubMed).

Ekert, Silke, Hawkins et al.: "DIABLO promotes apoptosis by removing MIHA/XIAP from processed caspase 9." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 483-90, 2001 (PubMed).

Srinivasula, Datta, Fan et al.: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, 2000 (PubMed).

Verhagen, Ekert, Pakusch et al.: "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins." in: Cell, Vol. 102, Issue 1, pp. 43-53, 2000 (PubMed).

Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).