Second Mitochondria-Derived Activator of Caspase (DIABLO) antibody

Details for Product No. ABIN371649
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Synonyms smac, smac3, diablo-s, 0610041G12Rik, 1700006L01Rik, AU040403, Smac, DFNA64, SMAC, DBO, XSmac
(150), (51), (31), (3), (3), (2), (2), (1), (1)
(136), (28), (7), (1)
Clonality (Clone)
Monoclonal ()
Enzyme Immunoassay (EIA), Western Blotting (WB)
(161), (56), (55), (37), (26), (8), (8), (7), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 6 references available
Quantity 0.1 mg
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Catalog No. ABIN371649
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Clone 2D12
Isotype IgG2b
Specificity This antibody is specific for Human SMAC/DIABLO.
Characteristics Synonyms: Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, DirectIAP-binding protein with low pI
Purification Purified
Alternative Name DIABLO / SMAC
Background SMAC/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. SMAC/DIABLO has been shown to neutralize the antiapoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis. (Ref.1-6)Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
UniProt Q9NR28
Application Notes ELISA: 1/1,000-1/2,000. Western Blot: 1/5,000-1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Hu, Yang: "Cellular inhibitor of apoptosis 1 and 2 are ubiquitin ligases for the apoptosis inducer Smac/DIABLO." in: The Journal of biological chemistry, Vol. 278, Issue 12, pp. 10055-60, 2003 (PubMed).

Adrain, Creagh, Martin: "Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2." in: The EMBO journal, Vol. 20, Issue 23, pp. 6627-36, 2001 (PubMed).

Ekert, Silke, Hawkins et al.: "DIABLO promotes apoptosis by removing MIHA/XIAP from processed caspase 9." in: The Journal of cell biology, Vol. 152, Issue 3, pp. 483-90, 2001 (PubMed).

Srinivasula, Datta, Fan et al.: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, 2000 (PubMed).

Verhagen, Ekert, Pakusch et al.: "Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins." in: Cell, Vol. 102, Issue 1, pp. 43-53, 2000 (PubMed).

Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

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