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AXIN1 antibody (Axin 1) (C-Term)

Details for Product anti-AXIN1 Antibody No. ABIN372934, Supplier: Log in to see
Antigen
  • AXIN1
  • axin
  • fused
  • xAxin
  • LOC100226467
  • AXIN
  • PPP1R49
  • AI316800
  • Axin
  • Fu
  • Kb
  • Ki
  • kinky
  • knobbly
  • AXIN1-RGS
  • wu:fi22e03
  • wu:fj14c08
  • wu:fk11b03
  • zgc:109872
Alternatives
anti-Human AXIN1 antibody for ELISA
Epitope
C-Term
47
16
15
15
13
10
4
4
3
3
2
2
2
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
111
34
26
4
4
2
1
1
1
1
1
Host
Goat
86
18
14
Clonality
Polyclonal
Conjugate
This AXIN1 antibody is un-conjugated
8
8
6
5
5
5
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
87
64
31
14
11
10
5
3
3
1
Supplier
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Immunogen Synthetic peptide from C-terminus of Human AXIN1.
Sequence c-FEEKIIGKVE KVD
Isotype IgG
Specificity This antibody recognizes Axin 1 (AXIN1).
Cross-Reactivity (Details) Species reactivity (expected):Canine (Dog), Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography.
Alternative Name Axin-1 (AXIN1 Antibody Abstract)
Background Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta-Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).Synonyms: AXIN, AXIN1, Axis inhibition protein 1
Gene ID 8312
NCBI Accession NP_003493
UniProt O15169
Pathways WNT Signaling
Application Notes ELISA: 1/28000. Immunohistochemistry on Paraffin Sections: 3.75 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris-Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-AXIN1 antibody (Axin 1) (C-Term) (ABIN372934) anti-Axin 1 (AXIN1) (C-Term) antibody
Background publications Zeng, Tamai, Doble et al.: "A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation." in: Nature, Vol. 438, Issue 7069, pp. 873-7, 2005 (PubMed).

Dalton: "Universities scramble to assess scope of falsified results." in: Nature, Vol. 438, Issue 7064, pp. 7, 2005 (PubMed).

Chia, Costantini: "Mouse axin and axin2/conductin proteins are functionally equivalent in vivo." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4371-6, 2005 (PubMed).

Salahshor, Woodgett: "The links between axin and carcinogenesis." in: Journal of clinical pathology, Vol. 58, Issue 3, pp. 225-36, 2005 (PubMed).

Luo, Lin: "Axin: a master scaffold for multiple signaling pathways." in: Neuro-Signals, Vol. 13, Issue 3, pp. 99-113, 2004 (PubMed).

Leung, Kolligs, Wu et al.: "Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling." in: The Journal of biological chemistry, Vol. 277, Issue 24, pp. 21657-65, 2002 (PubMed).

Lustig, Jerchow, Sachs et al.: "Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1184-93, 2002 (PubMed).

Jho, Zhang, Domon et al.: "Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1172-83, 2002 (PubMed).

Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).