Axin 1 (AXIN1) (C-Term) antibody

Details for Product No. ABIN372934
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Antigen
Synonyms AXIN1, axin, fused, xAxin, LOC100226467, AXIN, PPP1R49, AI316800, Axin, Fu, Kb, Ki, kinky, knobbly, AXIN1-RGS, wu:fi22e03, wu:fj14c08, wu:fk11b03, zgc:109872
Epitope
C-Term
(20), (8), (7), (7), (7), (4), (3), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(67), (26), (22), (15), (14), (12), (12), (1)
Host
Goat
(49), (15), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(51), (33), (10), (9), (9), (8), (8), (7), (1)
Pubmed 9 references available
Quantity 50 µg
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Catalog No. ABIN372934
500.50 $
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Immunogen Synthetic peptide from C-terminus of Human AXIN1.
Sequence c-FEEKIIGKVE KVD
Isotype IgG
Specificity This antibody recognizes Axin 1 (AXIN1).
Purification Immunoaffinity Chromatography.
Alternative Name Axin-1
Background Axin-1 (Axis inhibition protein 1) and Axin-2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin-1 interacts with APC, GSK3-beta, Dvl and beta- Catenin and promotes the GSK3-beta-mediated phosphorylation and subsequent degradation of beta-Catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute Axin1 in mice (6). Axin-2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).
Alternate names: AXIN, AXIN1, Axis inhibition protein 1
Gene ID 8312
NCBI Accession NP_003493
UniProt O15169
Application Notes ELISA: 1/28000. Immunohistochemistry on Paraffin Sections: 3.75 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris-Saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Axin 1 (AXIN1) (C-Term) antibody anti-Axin 1 (AXIN1) (C-Term) antibody
Background publications Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Jho, Zhang, Domon et al.: "Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a negative regulator of the signaling pathway." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1172-83, 2002 (PubMed).

Lustig, Jerchow, Sachs et al.: "Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors." in: Molecular and cellular biology, Vol. 22, Issue 4, pp. 1184-93, 2002 (PubMed).

Leung, Kolligs, Wu et al.: "Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling." in: The Journal of biological chemistry, Vol. 277, Issue 24, pp. 21657-65, 2002 (PubMed).

Luo, Lin: "Axin: a master scaffold for multiple signaling pathways." in: Neuro-Signals, Vol. 13, Issue 3, pp. 99-113, 2004 (PubMed).

Salahshor, Woodgett: "The links between axin and carcinogenesis." in: Journal of clinical pathology, Vol. 58, Issue 3, pp. 225-36, 2005 (PubMed).

Chia, Costantini: "Mouse axin and axin2/conductin proteins are functionally equivalent in vivo." in: Molecular and cellular biology, Vol. 25, Issue 11, pp. 4371-6, 2005 (PubMed).

Dalton: "Universities scramble to assess scope of falsified results." in: Nature, Vol. 438, Issue 7064, pp. 7, 2005 (PubMed).

Zeng, Tamai, Doble et al.: "A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation." in: Nature, Vol. 438, Issue 7069, pp. 873-7, 2005 (PubMed).

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