FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody

Details for Product No. ABIN374185
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Antigen
Synonyms FXYD5, DYSAD, IWU1, KCT1, OIT2, PRO6241, RIC, EF-8, Oit2
Epitope
C-Term
(8), (8), (5), (5), (3)
Reactivity
Human
(27)
Host
Goat
(22), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(26), (12), (9), (5), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374185
357.50 $
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Immunogen Peptide from C Terminus of the protein sequence according to NP_054883.3, NP_659003.1, NP_001158077.1 Genename: FXYD5
Sequence GKCRQLSRLC RNHCR
Specificity This antibody detects FXYD5 at C-term.
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name Dysadherin / FXYD5
Background Dysadherin (FXYD5) is a member of a family of small membrane proteins that share a 35aa signature sequence domain, beginning with the sequence PFXYD. This protein is a negative regulator of calcium-dependent cell-cell adhesion and is implicated in the biogenesis of microvilli.Synonyms: DYSAD, FXYD domain-containing ion transport regulator 5, IWU1
Gene ID 53827
NCBI Accession NP_054883
UniProt Q96DB9
Application Notes Peptide ELISA: Limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 38 kDa band in Human Spleenlysate and an approx 26 kDa in Human Lung lysate after 1 μg/mL antibody staining. Thebands were successfully blocked by incubation with the immunizing peptide. Please notethat according to the literature this protein is O-glycosylated (PMID: 12672699), which mayexplain the different sizes of bands we observe given the calculated size of 19.5 kDaaccording to NP_054883 and NP_659003. We would appreciate any feedback from peoplein the field - have any results been reported with other antibodies/lysates?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody anti-FXYD Domain Containing Ion Transport Regulator 5 (FXYD5) (C-Term) antibody
Background publications Ino, Gotoh, Sakamoto et al.: "Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 1, pp. 365-70, 2002 (PubMed).

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