HLTF antibody (C-Term)
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- Target See all HLTF Antibodies
- HLTF (Helicase-Like Transcription Factor (HLTF))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog, Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This HLTF antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-KQAKINEIRT LIDL
- Specificity
- This antibody detects RNF80 / HLTF at C-term.
- Cross-Reactivity (Details)
- Species reactivity (expected):Mouse, Rat, Dag, CowSpecies reactivity (tested):Human
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_003062.2, NP_620636.1 Genename: HLTF
- Top Product
- Discover our top product HLTF Primary Antibody
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anti-Helicase-Like Transcription Factor (HLTF) (AA 837-996) antibody
HLTF Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (AA 332-476) antibodyHLTF Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (AA 409-437) antibodyHLTF Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB20624 unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (AA 831-990) antibodyHLTF Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (C-Term) antibodyVerified HLTF Reactivity: Human WB, ELISA, FACS Host: Goat Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (N-Term) antibodyHLTF Reactivity: Human, Rat, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (C-Term) antibodyHLTF Reactivity: Human, Rat, Dog, Rabbit, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (N-Term) antibodyHLTF Reactivity: Human, Mouse, Dog, Rabbit, Cow, Horse, Pig, Monkey, Bat WB Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) (AA 726-1009) antibodyHLTF Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Helicase-Like Transcription Factor (HLTF) antibodyHLTF Reactivity: Human WB Host: Rabbit Monoclonal unconjugated
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- Application Notes
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-1 μg/mL. Approx 120 kDa and 50 kDa bands seen in Jurkat cell lysate. Please note that the weaker smaller band may represent a novel isoform of this proteinand is in agreement with previous Western blot observations (J Biol Chem 1995 Mar3,270(9): 4575-87).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- HLTF (Helicase-Like Transcription Factor (HLTF))
- Alternative Name
- SMARCA3 / HLTF (HLTF Products)
- Background
- Synonyms: DNA-binding protein/plasminogen activator inhibitor 1 regulator, HIP116, HIP116A, Helicase-like transcription factor, RING finger protein 80, RNF80, SNF2L3, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3, Sucrose nonfermenting protein 2-like 3, ZBU1
- Gene ID
- 6596
- NCBI Accession
- NP_003062
- UniProt
- Q14527
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