SMARCE1 antibody (C-Term)

Catalog No. ABIN374349

Product Details anti-SMARCE1 Antibody

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This SMARCE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-PPTDPIPEDE KKE

Specificity: This antibody detects SMARCE1 / BAF57 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_003070.3 Genename: SMARCE1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEPG2,HeLa and Jurkat (calculated MW of 46.0 kDa according to NP_003070.3). A 40 kDa band wasobserved in transfected HEK293 transiently expressing BAF57. This band is not observed inthe non-transfected HEK293. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EABIN374349 (mocktransfection in first lane), tested by Origene. B) ABIN374349 staining (0.2 μg/mL) of Jurkat lysate (RIPA buffer, 35 μg total protein perlane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SMARCE1

Target: SMARCE1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))

Alternative Name: SMARCE1 / BAF57 (SMARCE1 Products)

Synonyms: BAF57 antibody, XBaf57 antibody, MGC52696 antibody, MGC69429 antibody, MGC81726 antibody, SMARCE1 antibody, DKFZp459O2235 antibody, fb54d11 antibody, wu:fb33d02 antibody, wu:fb54d11 antibody, Baf57 antibody, 2810417B20Rik antibody, 5830412H02Rik antibody, 9030408N19Rik antibody, Baf27 antibody, RGD1304726 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 antibody, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 L homeolog antibody, smarce1 antibody, SMARCE1 antibody, smarce1.L antibody, Smarce1 antibody

Background: Synonyms: BRG1-associated factor 57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

Gene ID: 6605

NCBI Accession: NP_003070

UniProt: Q969G3

Pathways: Chromatin Binding