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Details for Product No. ABIN374349

SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1) (C-Term) antibody

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Antigen
Synonyms BAF57, Baf27, 2810417B20Rik, 5830412H02Rik, 9030408N19Rik, RGD1304726, fb54d11, wu:fb33d02, wu:fb54d11, Baf57, XBaf57, MGC52696, MGC69429, MGC81726, SMARCE1, DKFZp459O2235
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
Clonality Polyclonal
Application
»Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN374349
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Peptide from C Terminus of the protein sequence according to NP_003070.3 Genename: SMARCE1
Sequence C-PPTDPIPEDEKKE
Specificity This antibody detects SMARCE1 / BAF57 at C-term. Species Reactivity: Tested: Human Expected from sequence similarity: Mouse
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name SMARCE1 / BAF57
Background Alternate names: BRG1-associated factor 57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Gene ID 6605
NCBI Accession NP_003070.3
UniProt Q969G3
Research Area Apoptosis/Necrosis
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.6 µg/ml. Approx 50kDa band observed in lysates of cell lines HEPG2,HeLa and Jurkat (calculated MW of 46.6kDa according to NP_003070.3). A 48kDa band wasobserved in transfected HEK293 transiently expressing BAF57. This band is not observed inthe non-transfected HEK293. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EABIN374349 (mocktransfection in first lane), tested by Origene. B) ABIN374349 staining (0.2 µg/ml) of Jurkat lysate (RIPA buffer, 35 µg total protein perlane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Background publications Wang, Chi, Xue et al.: "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 492-8, 1998 (PubMed).

Alternatives for antigen "SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1)", type "Antibodies"
Hosts (19), (8), (4)
Reactivities (28), (7), (5), (2), (2), (2), (2), (2), (1)
Applications (30), (12), (10), (6), (4), (1), (1), (1), (1)
Epitopes (8), (4), (3), (2), (1), (1)
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