Applications: FC - Quality tested , IHC-FS - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 3 g/106 cells PE, APC, and SPRD conjugates 0.3 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Sample Volume
1 mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of PBS
Preservative
Azide free
Handling Advice
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage
-20 °C
Storage Comment
Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
CD24, also known as heat stable antigen, is a phosphatidylinositol-linked cell surface glycoprotein that is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4 + CD8 + CD24 + thymocytes to either CD4 + CD8 - CD24 - or CD4 - CD8 + CD24 - T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P-selectin (CD62P). 7 CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes.