HA-Tag antibody

Details for Product No. ABIN387747
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Antigen
Reactivity
Tag
(280), (14), (4), (3), (3), (3), (2), (1)
Host
Mouse
(137), (123), (23), (17), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(31), (20), (18), (6), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Western Blotting (WB)
(239), (101), (82), (69), (35), (21), (15), (15), (14), (14), (9), (8), (5), (4), (4), (3), (3), (3), (2), (2), (2)
Pubmed 17 references available
Quantity 400 μL
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Immunogen KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Clone 12CA5
Isotype IgG2b
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.

Synonyms: Tag from influenza hemagglutinin protein
Research Area Tags/Labels
Application Notes Suggested dilutions:
WB = 1:1, 000
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-HA-Tag antibody (TOP)HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate ...
Product cited in: Mertz, Tan, Pagala et al.: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, 2015 (PubMed).

Koo, Yue, Gal et al.: "Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth." in: Cancer research, Vol. 74, Issue 9, pp. 2555-68, 2014 (PubMed).

Bruntz, Taylor, Lindsley et al.: "Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 600-16, 2014 (PubMed).

Li, Ren, Yue et al.: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, 2012 (PubMed).

Sun, Ni: "HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation." in: Molecular plant, Vol. 4, Issue 1, pp. 116-26, 2011 (PubMed).

Satow, Shitashige, Jigami et al.: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, 2010 (PubMed).

Herrera, Silva, Garvin: "Angiotensin II stimulates thick ascending limb superoxide production via protein kinase C(α)-dependent NADPH oxidase activation." in: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21323-8, 2010 (PubMed).

Kauh, Fan, Xia et al.: "c-FLIP degradation mediates sensitization of pancreatic cancer cells to TRAIL-induced apoptosis by the histone deacetylase inhibitor LBH589." in: PLoS ONE, Vol. 5, Issue 4, pp. e10376, 2010 (PubMed).

Nishikawa, Nakashima, Hayashi et al.: "Blimp1-mediated repression of negative regulators is required for osteoclast differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 3117-22, 2010 (PubMed).

Czymai, Viemann, Sticht et al.: "FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10163-78, 2010 (PubMed).

Flozak, Lam, Russell et al.: "Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3157-67, 2010 (PubMed).

Guo, Thomas, Collins et al.: "Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis." in: The Plant cell, Vol. 20, Issue 1, pp. 48-58, 2008 (PubMed).

Choe, Liao, Zhou et al.: "Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 35, pp. 9503-12, 2007 (PubMed).

Chen, Liu, Yue et al.: "CCAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction and ubiquitin/proteasome-mediated cellular FLICE-inhibitory protein down-regulation contribute to enhancement of ..." in: Molecular pharmacology, Vol. 72, Issue 5, pp. 1269-79, 2007 (PubMed).

Hu, Straub, Xiao et al.: "Phenethyl isothiocyanate, a cancer chemopreventive constituent of cruciferous vegetables, inhibits cap-dependent translation by regulating the level and phosphorylation of 4E-BP1." in: Cancer research, Vol. 67, Issue 8, pp. 3569-73, 2007 (PubMed).

Adler, Lin, Horlings et al.: "Genetic regulators of large-scale transcriptional signatures in cancer." in: Nature genetics, Vol. 38, Issue 4, pp. 421-30, 2006 (PubMed).

Mamidipudi, Zhang, Lee et al.: "RACK1 regulates G1/S progression by suppressing Src kinase activity." in: Molecular and cellular biology, Vol. 24, Issue 15, pp. 6788-98, 2004 (PubMed).

Validation Images
Catalog No. ABIN387747
214.50 $
Plus shipping costs $45.00
Quantity
Price
400 μL
214.50 $

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