HA-Tag antibody

Details for Product No. ABIN387747
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(270), (14), (5), (5), (3), (3), (2), (1)
(136), (111), (30), (16), (2), (1)
Clonality (Clone)
Monoclonal ()
(30), (20), (16), (8), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Western Blotting (WB)
(234), (97), (87), (70), (34), (21), (15), (15), (14), (12), (9), (9), (8), (6), (5), (5), (4), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN387747
214.50 $
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Immunogen KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Clone 12CA5
Isotype IgG2b
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Target Type Tag
Background Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.

Synonyms: Tag from influenza hemagglutinin protein
Research Area Tags/Labels
Application Notes Suggested dilutions:
WB = 1:1, 000
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-HA-Tag antibody (TOP)HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody (ABIN387747). (Bottom) Western blot analysis of anti-HA Tag Antibody (SG071213A) in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.
General Czymai, Viemann, Sticht et al.: "FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10163-78, 2010 (PubMed).

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