HA-Tag antibody
| Antigen |
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| Clonality |
Monoclonal (12CA5) |
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Host
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Reactivity
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Conjugate
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Biotin (5), HRP (4), DyLight 488 (2), DyLight 650 (2), PerCP (2), SureLight® R-PE (2), APC (1), Alkaline Phosphatase (AP) (1), DyLight 550 (1), FITC (1), Peroxidase (POD) (1)
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Application
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Western Blotting (WB) (51), ELISA (34), Immunoprecipitation (IP) (28), Immunohistochemistry (IHC) (19), Neutralization (Neut) (12), Flow Cytometry (FACS) (11), Cellular Assay (CA) (9), Immunofluorescence (IF) (7), Dot Blot (Dot) (3), Immunomicroscopy (IM) (3), Immunocytochemistry (ICC) (2), Immunostaining (ISt) (2), Micro Array (2), Chromatin Immunoprecipitation (ChiP) (1), Fluorescence Microscopy (FM) (1)
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| Catalog no. |
ABIN387747 |
| Quantity |
0.1 mg (0.25 mg/ml) |
| Price |
214.50 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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HA Tag
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Immunogen
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KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
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Isotype
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IgG2b
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Clone
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12CA5
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Description
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Other names: Tag from influenza hemagglutinin protein
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Characteristics
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Mouse Monoclonal Antibody (Mab)
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Specificity
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KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
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Molecular Weight
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DA
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Comments
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Background: Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
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Application Notes
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ELISA~~1:5,000 Western blotting~~1:1,000
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Concentration
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0.25 mg/ml
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Buffer
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Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
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Storage
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Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Czymai, Viemann, Sticht et al.: "FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10163-78, 2010 (PubMed).
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Alternatives for antigen "HA-Tag", type "Antibodies"
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Hosts
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Mouse (45), Rabbit (22), Goat (4)
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Reactivities
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Tag (33), tag (13), All Species (7), Mouse (Murine) (3), Human (2), Rat (Rattus) (2), Various Species (1)
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Applications
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Western Blotting (WB) (51), ELISA (34), Immunoprecipitation (IP) (28), Immunohistochemistry (IHC) (19), Neutralization (Neut) (12), Flow Cytometry (FACS) (11), Cellular Assay (CA) (9), Immunofluorescence (IF) (7), Dot Blot (Dot) (3), Immunomicroscopy (IM) (3), Immunocytochemistry (ICC) (2), Immunostaining (ISt) (2), Micro Array (2), Chromatin Immunoprecipitation (ChiP) (1), Fluorescence Microscopy (FM) (1)
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Conjugates
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Biotin (5), HRP (4), DyLight 488 (2), DyLight 650 (2), PerCP (2), SureLight® R-PE (2), APC (1), Alkaline Phosphatase (AP) (1), DyLight 550 (1), FITC (1), Peroxidase (POD) (1)
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Epitopes
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C-Term (3), Internal Region (1)
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Alternatives 
