EPH Receptor B4 (EPHB4) antibody

Antigen: EPH Receptor B4 (EPHB4)

Synonyms: HTK, MYK1, TYRO11, htk, myk1, ephB4a, tyro11, ephb4b, MGC130997

Clonality: Monoclonal (67CT23-5-8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387814

Additional Information

Alternative name: EPHB4

Gene ID: 2050

UniProt: P54760

Immunogen: EPHB4 recombinant protein is used to produce this monoclonal antibody.

Isotype: IgG1 kappa

Clone: 67CT23-5-8

Description: Other names: Ephrin type-B receptor 4 , Tyrosine-protein kinase receptor HTK

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: EPHB4 recombinant protein is used to produce this monoclonal antibody.

Molecular Weight: 108269 DA

Comments:

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:10000 Western blotting~~ 1:200~2000

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Stem Cells, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-EPHB4 Monoclonal Antibody (Cat.#ABIN387814) by EPHB4 recombinant protein(Fragment). EPHB4 protein (arrow) was detected using the purified Mab.

Publications

Product: Pennisi, Ling, Li et al.: "The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth." in: Blood, Vol. 114, Issue 9, pp. 1803-12, 2009 (PubMed).

Kwan Tat, Pelletier, Amiable et al.: "Treatment with ephrin B2 positively impacts the abnormal metabolism of human osteoarthritic chondrocytes." in: Arthritis research & therapy, Vol. 11, Issue 4, pp. R119, 2009 (PubMed).

Suzuki, Abe, Imagama et al.: "BCR-ABL-independent and RAS / MAPK pathway-dependent form of imatinib resistance in Ph-positive acute lymphoblastic leukemia cell line with activation of EphB4." in: European journal of haematology, Vol. 84, Issue 3, pp. 229-38, 2010 (PubMed).

Weinsheimer, Kim, Pawlikowska et al.: "EPHB4 gene polymorphisms and risk of intracranial hemorrhage in patients with brain arteriovenous malformations." in: Circulation. Cardiovascular genetics, Vol. 2, Issue 5, pp. 476-82, 2009 (PubMed).

Kuang, Bai, Fang et al.: "Aberrant DNA methylation and epigenetic inactivation of Eph receptor tyrosine kinases and ephrin ligands in acute lymphoblastic leukemia." in: Blood, Vol. 115, Issue 12, pp. 2412-9, 2010 (PubMed).