Histone Acetyltransferase 1 (HAT1) (N-Term), (AA 27-58) antibody

Details for Product No. ABIN387931
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Antigen
Epitope
N-Term, AA 27-58
(19), (10), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(50), (10), (10), (1)
Host
Rabbit
(49), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB)
(50), (32), (6), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 400 µL
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Catalog No. ABIN387931
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Immunogen This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-58 AA from the N-terminal region of human KAT1 (HAT1).
Clone RB5768
Isotype Ig
Specificity This KAT1 (HAT1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35~65 amino acids from the N-terminal region of human HAT1.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name KAT1 (HAT1)
Background Histone acetylation, particularly of histone H4, has been proposed to play an important role in replication-dependent nucleosome assembly. The HAT1 protein contains D, A, and B motifs, which are present in many N-acetyltransferases, including those that acetylate substrates other than histones. The HAT1 holoenzyme consists of 2 subunits: the catalytic 46-kD HAT1 and the accessory p46. The p46 subunit stimulated the activity of HAT1 and bound to core histones. The HAT1 holoenzyme acetylated newly synthesized but not nucleosomal histone H4 at lys5 and lys12, and, to a lesser extent, histone H2A at lys5. HAT1 and p46 polypeptides are located in the nucleus of S-phase cells. HAT1 may play a role in telomeric silencing.
Synonyms: Histone acetyltransferase type B catalytic subunit, KAT1
Molecular Weight 49513 DA
Gene ID 8520
UniProt O14929
Research Area Signaling, Protein Modifications, Cancer, Cell Cycle, Chromatin, Cell Structure
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Histone Acetyltransferase 1 (HAT1) (N-Term), (AA 27-58) antibody Western blot analysis of KAT1 (HAT1) Antibody (N-term) (ABIN387931) in HL60 cell line lysates (35 µg/lane). HAT1 (arrow) was detected using the purified polyclonal antibody.
Background publications Cheung, Tanner, Cheung et al.: "Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation." in: Molecular cell, Vol. 5, Issue 6, pp. 905-15, 2000 (PubMed).

Makowski, Dutnall, Annunziato: "Effects of acetylation of histone H4 at lysines 8 and 16 on activity of the Hat1 histone acetyltransferase." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 43499-502, 2001 (PubMed).

Grönroos, Hellman, Heldin et al.: "Control of Smad7 stability by competition between acetylation and ubiquitination." in: Molecular cell, Vol. 10, Issue 3, pp. 483-93, 2002 (PubMed).

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