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SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (N-Term), (AA 25-53) antibody

Details for Product No. ABIN387992
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Antigen
Synonyms KMT5A, PR-Set7, SET07, SET8, 2410195B05Rik, AA617402, AW536475, PR-SET7
Epitope
N-Term, AA 25-53
(28), (8), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(52), (10), (1)
Host
Rabbit
(29), (23)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (5), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(47), (34), (20), (10), (7), (4), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN387992
291.50 $
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Immunogen This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-53 AA from the N-terminal region of human SET07.
Clone RB1415
Isotype Ig
Specificity This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~62 amino acids from the N-terminal region of human SET07.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SET07
Background SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
Synonyms: Histone-lysine N-methyltransferase, H4 lysine-20 specific, Histone H4-K20 methyltransferase, H4-K20-HMTase, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR-Set7 , PR/SET07, KMT5A
Molecular Weight 42890 DA
Gene ID 387893
UniProt Q9NQR1
Research Area Signaling, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (N-Term), (AA 25-53) antibody Western blot analysis of SET07 polyclonal antibody (ABIN387992) in HeLa cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2). SET07 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (N-Term), (AA 25-53) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Nishioka, Rice, Sarma et al.: "PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin." in: Molecular cell, Vol. 9, Issue 6, pp. 1201-13, 2002 (PubMed).

Validation Images
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