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BNIP3 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 3) (AA 152-187)

Details for Product anti-BNIP3 Antibody No. ABIN388117, Supplier: Login to see New
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Synonyms NIP3, Nip3
AA 152-187, BH3 Domain, N-Term
(24), (22), (14), (11), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(119), (53), (25)
(114), (5), (2)
Clonality (Clone)
Polyclonal ()
This BNIP3 antibody is un-conjugated
(5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(92), (28), (25), (22), (17), (14), (11), (9), (2), (2), (1)
Pubmed 5 references available
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Quantity 400 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Immunogen This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 AA from human BNIP3.
Clone RB3385
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name BNIP3 (NIP3) (BNIP3 Antibody Abstract)
Background NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

Synonyms: BCL2/adenovirus E1B 19-kDa protein-interacting protein 3, BNIP3
Molecular Weight 21541 Da
Gene ID 664
UniProt Q12983
Research Area Apoptosis/Necrosis, Cell Structure, Cancer
Application Notes Suggested dilutions:
IF = 1:50-100, WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BNIP3 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 3) (AA 152-187) (ABIN388117) The anti-NIP3 BH3 domain Pab (ABIN388117) is used in Western blot to detect NIP3 BH3 ...
Immunohistochemistry (IHC) image for anti-BNIP3 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 3) (AA 152-187) (ABIN388117) Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass cov...
Immunohistochemistry (IHC) image for anti-BNIP3 antibody (BCL2/adenovirus E1B 19kDa Interacting Protein 3) (AA 152-187) (ABIN388117) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Karpathiou, Sivridis, Koukourakis et al.: "Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 121, Issue 7, pp. 592-604, 2013 (PubMed).

Wojtkowiak, Rothberg, Kumar et al.: "Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments." in: Cancer research, Vol. 72, Issue 16, pp. 3938-47, 2012 (PubMed).

Metukuri, Beer-Stolz, Namas et al.: "Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 296, Issue 3, pp. G499-509, 2009 (PubMed).

Background publications Kothari, Cizeau, McMillan-Ward et al.: "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF." in: Oncogene, Vol. 22, Issue 30, pp. 4734-44, 2003 (PubMed).

Yasuda, Theodorakis, Subramanian et al.: "Adenovirus E1B-19K/BCL-2 interacting protein BNIP3 contains a BH3 domain and a mitochondrial targeting sequence." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12415-21, 1998 (PubMed).

Catalog No. ABIN388117
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