BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 152-187), (N-Term), (BH3 Domain) antibody

Details for Product No. ABIN388117
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Antigen
Synonyms NIP3, Nip3
Epitope
AA 152-187, N-Term, BH3 Domain
(12), (7), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(61), (29), (26), (24), (24), (13), (12)
Host
Rabbit
(61), (4), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
(41), (20), (18), (16), (11), (9), (8), (8), (3), (2), (1)
Pubmed 2 references available
Catalog no. ABIN388117
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Immunogen This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-187 AA from human BNIP3.
Clone RB3385
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name BNIP3 (NIP3)
Background NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

Synonyms: BCL2/adenovirus E1B 19-kDa protein-interacting protein 3, BNIP3
Molecular Weight 21541 Da
Gene ID 664
UniProt Q12983
Research Area Apoptosis/Necrosis, Cell Structure, Cancer
Application Notes Suggested dilutions:
IF = 1:50-100, WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 152-187), (N-Term), (BH3 Domain) antibody The anti-NIP3 BH3 domain Pab (ABIN388117) is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 152-187), (N-Term), (BH3 Domain) antibody (2) Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody ABIN388117. Data courtesy of Ruben Zamora, University of Pittsburgh.
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 152-187), (N-Term), (BH3 Domain) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Yasuda, Theodorakis, Subramanian et al.: "Adenovirus E1B-19K/BCL-2 interacting protein BNIP3 contains a BH3 domain and a mitochondrial targeting sequence." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12415-21, 1998 (PubMed).

Kothari, Cizeau, McMillan-Ward et al.: "BNIP3 plays a role in hypoxic cell death in human epithelial cells that is inhibited by growth factors EGF and IGF." in: Oncogene, Vol. 22, Issue 30, pp. 4734-44, 2003 (PubMed).

Hosts (61), (4), (1)
Reactivities (61), (29), (26), (24), (24), (13), (12)
Applications (41), (20), (18), (16), (11), (9), (8), (8), (3), (2), (1)
Conjugates (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (12), (7), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1)
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