BNIP3 antibody (AA 215-252)

Catalog No. ABIN388117

Product Details anti-BNIP3 Antibody

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Binding Specificity: AA 215-252

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This BNIP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-252 amino acids from human BNIP3.

Clone: RB3385

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:500. IF: 1:50~100. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-BNIP3 antibody (ABIN388117)

Karpathiou, Sivridis, Koukourakis, Mikroulis, Bouros, Froudarakis, Bougioukas, Maltezos, Giatromanolaki: "Autophagy and Bcl-2/BNIP3 death regulatory pathway in non-small cell lung carcinomas." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 121, Issue 7, pp. 592-604, (2013) (PubMed).

Wojtkowiak, Rothberg, Kumar, Schramm, Haller, Proemsey, Lloyd, Sloane, Gillies: "Chronic autophagy is a cellular adaptation to tumor acidic pH microenvironments." in: Cancer research, Vol. 72, Issue 16, pp. 3938-47, (2012) (PubMed).

Metukuri, Beer-Stolz, Namas, Dhupar, Torres, Loughran, Jefferson, Tsung, Billiar, Vodovotz, Zamora: "Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 296, Issue 3, pp. G499-509, (2009) (PubMed).

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP3 (NIP3) (BNIP3 Products)

Background: NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

Molecular Weight: 27832

Gene ID: 664

NCBI Accession: NP_004043

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation