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Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term), (AA 111-140) antibody

Details for Product No. ABIN388843
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Antigen
Synonyms SFT, UBCH5, UBC4/5, UBCH5A, E2(17)KB1, ube2d1, wu:fc16h06, zgc:73096, sft, ubch5, ubc4/5, ubch5a, e2(17)kb1
Epitope
C-Term, AA 111-140
(9), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(35), (18), (16), (13), (1), (1), (1), (1), (1)
Host
Rabbit
(32), (4)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(27), (17), (13), (10), (9), (1)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN388843
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 AA from the C-terminal region of human UBE2D1.
Clone RB04139
Isotype Ig
Specificity This UBE2D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~147 amino acids from the C-terminal region of human UBE2D1.
Predicted Reactivity Cow (Bovine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name UBE2D1
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Synonyms: Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase, Ubiquitin carrier protein, E2(17)KB 1
Molecular Weight 16602 DA
Gene ID 7321
UniProt NP_003329
Research Area Ubiquitin-related antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term), (AA 111-140) antibody Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in mouse cerebellum tissue lysates (35 µg/lane). UBE2D1 (arrow) was detected using the purified polyclonal antibody.
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term), (AA 111-140) antibody (2) Western blot analysis of anti-UBE2D1 Pab (ABIN388843) in, left-to-right, wild-type T3 cells (mouse mouse fibroblast cell line), wild-type T3 cells plus recombinant mouse UBE2D1(120 ng), and Hela cells. Electrophoresis performed on a 10% Bis-Tris gel. Membrane blocking with 10% milk-PBST. Blotting at 1:500 dilution of primary antibody. Data courtesy of Xiaoli Wang, MD. PhD. , Washington University School of Medicine.
anti-Ubiquitin-Conjugating Enzyme E2D 1 (UBE2D1) (C-Term), (AA 111-140) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Jensen, Bates, Yang et al.: "Identification of a family of closely related human ubiquitin conjugating enzymes." in: The Journal of biological chemistry, Vol. 270, Issue 51, pp. 30408-14, 1996 (PubMed).

Kamura, Sato, Iwai et al.: "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10430-5, 2000 (PubMed).

Gehrke, Riedel, Herrmann et al.: "UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis." in: Blood, Vol. 101, Issue 8, pp. 3288-93, 2003 (PubMed).

Brès, Kiernan, Linares et al.: "A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter." in: Nature cell biology, Vol. 5, Issue 8, pp. 754-61, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN388843
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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