ABCB5 antibody (N-Term)

Catalog No. ABIN390068

Product Details anti-ABCB5 Antibody

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Binding Specificity: AA 1-30, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.

Clone: RB16781

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000. WB: 1:2000. WB: 1:1000. FC: 1:25. FC: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-ABCB5 antibody (ABIN390068)

Ksander, Kolovou, Wilson, Saab, Guo, Ma, McGuire, Gregory, Vincent, Perez, Cruz-Guilloty, Kao, Call, Tucker, Zhan, Murphy, Lathrop, Alt, Mortensen, Lin, Zieske, Frank, Frank: "ABCB5 is a limbal stem cell gene required for corneal development and repair." in: Nature, Vol. 511, Issue 7509, pp. 353-7, (2014) (PubMed).

Freeman, Gray, Millward, Pearce, Ziman: "Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells." in: Journal of translational medicine, Vol. 10, pp. 192, (2013) (PubMed).

Luo, Ellis, Dallaglio, Takeda, Robinson, Robinson, Liu, Lewis, McCarter, Gonzalez, Norris, Roop, Spritz, Ahn, Fujita: "Side population cells from human melanoma tumors reveal diverse mechanisms for chemoresistance." in: The Journal of investigative dermatology, Vol. 132, Issue 10, pp. 2440-50, (2012) (PubMed).

Lai, Schwartz, Hsu: "CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry." in: Cancer research, Vol. 72, Issue 19, pp. 5111-8, (2012) (PubMed).

Wilson, Schatton, Zhan, Gasser, Ma, Saab, Schanche, Waaga-Gasser, Gold, Huang, Murphy, Frank, Frank: "ABCB5 identifies a therapy-refractory tumor cell population in colorectal cancer patients." in: Cancer research, Vol. 71, Issue 15, pp. 5307-16, (2011) (PubMed).

Ramgolam, Lauriol, Lalou, Lauden, Michel, de la Grange, Khatib, Aoudjit, Charron, Alcaide-Loridan, Al-Daccak: "Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function." in: PLoS ONE, Vol. 6, Issue 4, pp. e18784, (2011) (PubMed).

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ABCB5 (ABCB5 Products)

Background: ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Molecular Weight: 138641

Gene ID: 340273

NCBI Accession: NP_001157413, NP_001157414, NP_001157465, NP_848654

UniProt: Q2M3G0