Melanoma Antigen Family A, 3 (MAGEA3) (C-Term), (AA 283-314) antibody

Details for Product No. ABIN390107
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Antigen
Synonyms Mage-a3, MAGEA3, CT1.3, HIP8, HYPD, MAGE3, MAGEA6
Epitope
C-Term, AA 283-314
(9), (6), (3), (3), (3), (2), (1), (1), (1)
Reactivity
Human
(35)
Host
Rabbit
(20), (15)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
(31), (16), (10), (8), (8), (4), (2)
Pubmed 4 references available
Quantity 400 µL
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Catalog No. ABIN390107
291.50 $
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Immunogen This MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 AA from the C-terminal region of human MAGEA3.
Clone RB2084
Isotype Ig
Specificity This MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285~314 amino acids from the C-terminal region of human MAGEA3.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MAGEA3
Background MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
Synonyms: Melanoma-associated antigen 3, MAGE-3 antigen, Antigen MZ2-D, MAGE3
Molecular Weight 34747 DA
Gene ID 4102
UniProt P43357
Research Area Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, FACS = 1:10-50, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Melanoma Antigen Family A, 3 (MAGEA3) (C-Term), (AA 283-314) antibody The anti-MAGEA3 C-term Antibody (ABIN390107) is used in Western blot to detect MAGEA3 in Jurkat lysate.
anti-Melanoma Antigen Family A, 3 (MAGEA3) (C-Term), (AA 283-314) antibody (2) hMAGEA3-H298 Antibody (ABIN390107) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Melanoma Antigen Family A, 3 (MAGEA3) (C-Term), (AA 283-314) antibody (3) MAGEA3 Antibody (C-term) (ABIN390107)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Background publications van der Bruggen, Traversari, Chomez et al.: "A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma." in: Science (New York, N.Y.), Vol. 254, Issue 5038, pp. 1643-7, 1992 (PubMed).

Rogner, Wilke, Steck et al.: "The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28." in: Genomics, Vol. 29, Issue 3, pp. 725-31, 1996 (PubMed).

Consogno, Manici, Facchinetti et al.: "Identification of immunodominant regions among promiscuous HLA-DR-restricted CD4+ T-cell epitopes on the tumor antigen MAGE-3." in: Blood, Vol. 101, Issue 3, pp. 1038-44, 2003 (PubMed).

Guo, Wen, Huang et al.: "Detection of multiple melanoma-associated markers in melanoma cell lines by RT in situ PCR." in: Experimental and molecular pathology, Vol. 74, Issue 2, pp. 140-7, 2003 (PubMed).

Validation Images
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