SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 220-249), (Center) antibody

Details for Product No. ABIN390472
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Antigen
Epitope
AA 220-249, Center
(29), (8), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(53), (11), (1)
Host
Rabbit
(30), (23)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (5), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(48), (34), (21), (10), (7), (4), (1)
Pubmed 2 references available
Quantity 400 μL
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Catalog No. ABIN390472
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Immunogen This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 AA from the Central region of human SETD8.
Clone RB18964
Isotype Ig
Specificity This SETD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227~257 amino acids from the Center region of human SETD8.
Predicted Reactivity Cow (Bovine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SETD8
Background SETD8 is a histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.
Synonyms: Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, PR/SET07, PR-Set7, Lysine N-methyltransferase 5A, SET8, SET07, PRSET7, KMT5A, SETD8
Molecular Weight 42890 DA
Gene ID 387893
UniProt Q9NQR1
Research Area Signaling, Protein Modifications
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 220-249), (Center) antibody Western blot analysis of SETD8 antibody (Center) (ABIN390472) in 293 cell line lysates (35 µg/lane). SETD8 (arrow) was detected using the purified polyclonal antibody.
anti-SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 220-249), (Center) antibody (2) SETD8 Antibody (Center) (ABIN390472) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining
anti-SET Domain Containing (Lysine Methyltransferase) 8 (SETD8) (AA 220-249), (Center) antibody (3) SETD8 Antibody (Center) (ABIN390472) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Kalakonda, Fischle, Boccuni et al.: "Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1." in: Oncogene, Vol. 27, Issue 31, pp. 4293-304, 2008 (PubMed).

Houston, McManus, Adams et al.: "Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability." in: The Journal of biological chemistry, Vol. 283, Issue 28, pp. 19478-88, 2008 (PubMed).

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