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Calreticulin (CALR) (AA 277-305), (Center) antibody

Details for Product No. ABIN390885
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Antigen
Synonyms
crc, crt, zgc:85636, CG9429, Clr, Dmel\\CG9429, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, cc1qr, ssa, NV19007, CRT, RO, SSA, cC1qR, Calregulin, CRP55 ... show more
crc, crt, zgc:85636, CG9429, Clr, Dmel\\CG9429, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, cc1qr, ssa, NV19007, CRT, RO, SSA, cC1qR, Calregulin, CRP55, ERp60, HACBP, CALR show less
Epitope
AA 277-305, Center
(15), (6), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(133), (66), (47), (27), (20), (20), (19), (12), (9), (8), (6), (6), (1), (1), (1)
Host
Rabbit
(106), (26), (7), (5), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(133), (56), (55), (36), (27), (26), (23), (20), (12), (10), (1), (1)
Pubmed 1 reference available
Quantity 400 µL
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Catalog No. ABIN390885
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Immunogen This CALR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 AA from the Central region of human CALR.
Clone RB21112
Isotype Ig
Specificity This CALR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284~313 amino acids from the Center region of human CALR.
Predicted Reactivity Monkey
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name CALR
Background Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
Synonyms: Calreticulin, CRP55, Calregulin, HACBP, ERp60, grp60, CRTC, CALR
Molecular Weight 48142 DA
Gene ID 811
UniProt P27797
Research Area Neurology
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Calreticulin (CALR) (AA 277-305), (Center) antibody Western blot analysis of CALR Antibody (Center) (ABIN390885) in Ramos, Jurkat, HepG2, NIH-3T3 cell line lysates (35 µg/lane). CALR (arrow) was detected using the purified polyclonal antibody.
anti-Calreticulin (CALR) (AA 277-305), (Center) antibody (2) Formalin-fixed and paraffin-embedded human colon carcinoma reacted with CALR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Calreticulin (CALR) (AA 277-305), (Center) antibody (3) CALR Antibody (Center) (ABIN390885) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Alur, Nguyen, Eggener et al.: "Suppressive roles of calreticulin in prostate cancer growth and metastasis." in: The American journal of pathology, Vol. 175, Issue 2, pp. 882-90, 2009 (PubMed).

Validation Images
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