You are viewing an incomplete version of our website. Please click to reload the website as full version.

Protein Kinase D3 (PRKD3) (AA 352-384) antibody

Details for Product No. ABIN391014, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms PRKD3, EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU, 4930557O20Rik, 5730497N19Rik, Pkcnu, Prkcn
AA 352-384
(23), (17), (15), (14), (13), (13), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(105), (41), (14)
(105), (7), (1)
Clonality (Clone)
Polyclonal ()
(8), (8), (8), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(82), (54), (51), (22), (22), (19), (15), (3), (1)
Pubmed 4 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 400 μL
Shipping to United States ( )
Availability Will be delivered in 2 to 4 Business Days
Immunogen This PKC-nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-384 AA from human PKC-nu.
Clone RB1047
Isotype Ig
Specificity This PKC nu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361~390 amino acids from the center region of human PKC nu.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name PKC nu (PRKD3 Antibody Abstract)
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Synonyms: Protein kinase C, nu type, nPKC-nu, Protein kinase EPK2, PKD3
Molecular Weight 100470 Da
Gene ID 23683
UniProt O94806
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 1.2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase D3 (PRKD3) (AA 352-384) antibody (ABIN391014) Western blot analysis of anti-PKCnu Pab (ABIN391014) in lysate of HL60 cells stimulat...
Western Blotting (WB) image for anti-Protein Kinase D3 (PRKD3) (AA 352-384) antibody (ABIN391014) Upper panel, western blot analysis of GFP fusion protein expression in Panc-1 cells b...
Immunohistochemistry (IHC) image for anti-Protein Kinase D3 (PRKD3) (AA 352-384) antibody (ABIN391014) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Product cited in: Chen, Deng, Singh et al.: "Protein kinase D3 (PKD3) contributes to prostate cancer cell growth and survival through a PKCepsilon/PKD3 pathway downstream of Akt and ERK 1/2." in: Cancer research, Vol. 68, Issue 10, pp. 3844-53, 2008 (PubMed).

Background publications Yeaman, Ayala, Wright et al.: "Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network." in: Nature cell biology, Vol. 6, Issue 2, pp. 106-12, 2004 (PubMed).

Rey, Yuan, Young et al.: "Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonists." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23773-85, 2003 (PubMed).

Matthews, Dayalu, Thompson et al.: "Regulation of protein kinase Cnu by the B-cell antigen receptor." in: The Journal of biological chemistry, Vol. 278, Issue 11, pp. 9086-91, 2003 (PubMed).

Catalog No. ABIN391014
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)