LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN391033
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Antigen
Synonyms Lats, si:dkey-181i3.5, wu:fc19a11, zgc:123219, lats1, AW208599, WARTS, wts, RGD1564085
Epitope
N-Term, AA 1-30
(12), (12), (9), (7), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(58), (43), (43), (24), (24), (24), (12)
Host
Rabbit
(57), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(32), (30), (14), (12), (8), (2), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN391033
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Immunogen This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human LATS1.
Clone RB3029
Isotype Ig
Specificity This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human LATS1.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LATS1
Background The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.
Synonyms: Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, WARTS
Molecular Weight 126869 DA
Gene ID 9113
UniProt O95835
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1) (N-Term), (AA 1-30) antibody Western blot analysis of anti-LATS1 Antibody (N-term) (ABIN391033) in K562 cell line lysates (35 µg/lane). LATS1 (arrow) was detected using the purified polyclonal antibody.
anti-LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1) (N-Term), (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Hirota, Morisaki, Nishiyama et al.: "Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor." in: The Journal of cell biology, Vol. 149, Issue 5, pp. 1073-86, 2000 (PubMed).

Hisaoka, Tanaka, Hashimoto: "Molecular alterations of h-warts/LATS1 tumor suppressor in human soft tissue sarcoma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 82, Issue 10, pp. 1427-35, 2002 (PubMed).

Kamikubo, Takaori-Kondo, Uchiyama et al.: "Inhibition of cell growth by conditional expression of kpm, a human homologue of Drosophila warts/lats tumor suppressor." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17609-14, 2003 (PubMed).

Iida, Hirota, Morisaki et al.: "Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and G1 tetraploidy checkpoint function." in: Oncogene, Vol. 23, Issue 31, pp. 5266-74, 2004 (PubMed).

Yang, Yu, Hao et al.: "LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1." in: Nature cell biology, Vol. 6, Issue 7, pp. 609-17, 2004 (PubMed).

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