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Sphingosine Kinase 2 (SPHK2) (C-Term), (AA 590-620) antibody

Details for Product No. ABIN391349
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Antigen
Epitope
C-Term, AA 590-620
(13), (10), (5), (3), (3), (1)
Reactivity
Human
(72), (36), (26)
Host
Rabbit
(72), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(49), (42), (24), (19), (14), (3), (2), (1)
Pubmed 2 references available
Quantity 400 µL
Options
Shipping to United States (Change)
Availability Backorder
Catalog No. ABIN391349
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 AA from the C-terminal region of human SPHK2.
Clone RB03625
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name SPHK2
Background Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Synonyms: Sphingosine kinase 2, SK 2, SPK 2
UniProt Q9NRA0
Research Area Cancer, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Cell Cycle
Application Notes Suggested dilutions:
WB = 1:1000, IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-Sphingosine Kinase 2 (SPHK2) (C-Term), (AA 590-620) antibody The anti-SphK2 Pab (ABIN391349) is used in Western blot (Lane 1) to detect c-myc-tagged SphK2 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 2). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
anti-Sphingosine Kinase 2 (SPHK2) (C-Term), (AA 590-620) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Sphingosine Kinase 2 (SPHK2) (C-Term), (AA 590-620) antibody (3) SPHK2 Antibody (A605) (ABIN391349) western blot analysis in HepG2 cell line lysates (35 µg/lane).This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).
Background publications Shutler, Glassco, Kang et al.: "Genomic structure of the human D-site binding protein (DBP) gene." in: Genomics, Vol. 34, Issue 3, pp. 334-9, 1996 (PubMed).

Liu, Sugiura, Nava et al.: "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform." in: The Journal of biological chemistry, Vol. 275, Issue 26, pp. 19513-20, 2000 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN391349
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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