Homeodomain Interacting Protein Kinase 3 (HIPK3) (C-Term), (AA 1127-1156) antibody

Details for Product No. ABIN391779
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Antigen
Epitope
C-Term, AA 1127-1156
(6), (6), (3), (2), (1), (1), (1)
Reactivity
Human
(33), (13), (13), (13), (11), (11), (1), (1)
Host
Rabbit
(32), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(16), (15), (12), (9), (8), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN391779
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Immunogen This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 AA from the C-terminal region of human HIPK3.
Clone RB3241
Isotype Ig
Specificity This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1134~1163 amino acids from the sequence of human HIPK3 within a 1100-1200.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name HIPK3
Background HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.
Synonyms: Homeodomain-interacting protein kinase 3, Homolog of protein kinase YAK1, Fas-interacting serine/threonine-protein kinase, FIST, Androgen receptor-interacting nuclear protein kinase, ANPK, DYRK6, FIST3, PKY
Molecular Weight 133743 DA
Gene ID 10114
UniProt Q9H422
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Homeodomain Interacting Protein Kinase 3 (HIPK3) (C-Term), (AA 1127-1156) antibody Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and DYKDDDDK-sHIPK3 (AA 159-1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-DYKDDDDK antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and DYKDDDDK-sHIPK3.
anti-Homeodomain Interacting Protein Kinase 3 (HIPK3) (C-Term), (AA 1127-1156) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

General Tiefenbach, Moll, Nelson et al.: "A live zebrafish-based screening system for human nuclear receptor ligand and cofactor discovery." in: PLoS ONE, Vol. 5, Issue 3, pp. e9797, 2010 (PubMed).

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