HIPK3 antibody (Internal Region)

Catalog No. ABIN6262243

The Rabbit Polyclonal anti-HIPK3 antibody (ABIN6262243) specifically detects HIPK3 in WB, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for HIPK3 antibody (Internal Region) (ABIN6262243)

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-HIPK3 Antibody

Binding Specificity: Internal Region

Purpose: HIPK3 Antibody

Specificity: HIPK3 Antibody detects endogenous levels of total HIPK3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HIPK3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HIPK3

Target: HIPK3 (Homeodomain Interacting Protein Kinase 3 (HIPK3))

Alternative Name: HIPK3

Background:

Description: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

Gene: HIPK3

Molecular Weight: 134 kDa

Gene ID: 10114

UniProt: Q9H422