Bromodomain Containing 2 (BRD2) (AA 170-200), (Center) antibody

Details for Product No. ABIN392609
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Antigen
Synonyms D6S113E, FSH, FSRG1, NAT, RING3, RNF3, AW228947, D17H6S113E, Frg-1, Fsrg-1, Fsrg1, Nat, Ring3, Rnf3, mKIAA4005, brd2-a, fsh, fsrg1, nat, ring3, rnf3, brd2, fe47f02, fi85h05, wu:fe47f02, wu:fi85h05
Epitope
AA 170-200, Center
(8), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(51), (16), (15), (14), (14), (14), (14), (1), (1)
Host
Rabbit
(41), (8), (3)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(40), (13), (10), (9), (8), (6), (4), (4), (4), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 400 μL
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Catalog No. ABIN392609
291.50 $
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Immunogen This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 AA from the Central region of human BRD2.
Clone RB3416
Isotype Ig
Specificity This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178~207 amino acids from the center region of human BRD2.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name BRD2
Background BRD2 is a mitogen-activated kinase which localizes to the nucleus. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3 but sequence comparison suggests that the protein is not involved in the immune response. Homology to the Drosophila gene female sterile homeotic suggests that this human protein may be part of a signal transduction pathway involved in growth control.
Synonyms: Bromodomain-containing protein 2, RING3 protein
Molecular Weight 88060 DA
Gene ID 6046
UniProt P25440
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Bromodomain Containing 2 (BRD2) (AA 170-200), (Center) antibody BRD2-Q185 (ABIN392609) western blot analysis in 293 cell line lysates (35 µg/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).
anti-Bromodomain Containing 2 (BRD2) (AA 170-200), (Center) antibody (2) Western blot analysis of BRD2 (arrow) using rabbit polyclonal BRD2 Antibody (Center) (ABIN392609). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2) (Origene Technologies).
anti-Bromodomain Containing 2 (BRD2) (AA 170-200), (Center) antibody (3) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Crowley, Kaine, Yoshida et al.: "Reproductive cycle regulation of nuclear import, euchromatic localization, and association with components of Pol II mediator of a mammalian double-bromodomain protein." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 8, pp. 1727-37, 2002 (PubMed).

Validation Images
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