Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 3-33) antibody

Details for Product No. ABIN392627
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Antigen
Epitope
N-Term, AA 3-33
(20), (9), (8), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(122), (46), (12), (7), (3), (1)
Host
Rabbit
(69), (53), (9)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(115), (59), (43), (36), (28), (14), (13), (12), (10), (7), (4), (3), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 400 μL
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Catalog No. ABIN392627
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Immunogen This BUBR1 (BUB1B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-33 AA from the N-terminal region of human BUBR1 (BUB1B).
Clone RB03121-03122
Isotype Ig
Specificity This BUBR1 (BUB1B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11~40 amino acids from the N-terminal region of human BUB1B.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name BUBR1 (BUB1B)
Background BUB1B, a member of the Ser/Thr protein kinase family, is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. It can interact with BUB3, CENP-F, CENP-E and mitosin, and can be localized to nuclear kinetochores. This protein is highly expressed in thymus and spleen. The CD1 domain directs kinetochore localization and binding to BUB3. The protein possesses a cyclin destruction box sequence, which targets protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase. Defects in BUB1B are associated with tumor formation.
Synonyms: Mitotic checkpoint serine/threonine-protein kinase BUB1, hBub1, BUB1A
Molecular Weight 119517 DA
Gene ID 701
UniProt O60566
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 3-33) antibody Western blot analysis of BUB1B (arrow) using rabbit polyclonal BUB1B Antibody (N-term) (ABIN392627). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).
anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (N-Term), (AA 3-33) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Cahill, Lengauer, Yu et al.: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, 1998 (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, 1998 (PubMed).

Davenport, Fernandes, Harris et al.: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, 1999 (PubMed).

Cahill, da Costa, Carson-Walter et al.: "Characterization of MAD2B and other mitotic spindle checkpoint genes." in: Genomics, Vol. 58, Issue 2, pp. 181-7, 1999 (PubMed).

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