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U2AF Homology Motif (UHM) Kinase 1 (UHMK1) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN392641
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Antigen
Synonyms KIST, P-CIP2, 4732477C12Rik, 4930500M09Rik, AA673513, AI449218, AU021979, KIS, Kist, UHMK1, zgc:153241
Epitope
N-Term, AA 1-30
(17), (16), (4), (3), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(41), (29), (17), (13), (12), (2), (1)
Host
Rabbit
(41), (8), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(39), (25), (11), (10), (6), (2), (1), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN392641
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 AA from the N-terminal region of human KIST (KIS).
Clone RB3367
Isotype Ig
Specificity This KIS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human KIS.
Predicted Reactivity Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name KIST (KIS)
Background Upon serum stimulation, KIS, a member of the Ser/Thr protein kinase family, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. This protein, which contains 1 RRM (RNA recognition motif)domain, may be involved in trafficking and/or processing of RNA. KIS is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes.
Synonyms: Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, U2AF homology motif kinase 1, UHMK1
Molecular Weight 46546 DA
Gene ID 127933
UniProt Q8TAS1
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-U2AF Homology Motif (UHM) Kinase 1 (UHMK1) (N-Term), (AA 1-30) antibody Western blot analysis of anti-KIS Pab (ABIN392641) in mouse heart tissue lysate. KIS (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-U2AF Homology Motif (UHM) Kinase 1 (UHMK1) (N-Term), (AA 1-30) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-U2AF Homology Motif (UHM) Kinase 1 (UHMK1) (N-Term), (AA 1-30) antibody (3) KIS Antibody (C6) (ABIN392641) western blot analysis in A549 cell line lysates (35 µg/lane).This demonstrates the KIS antibody detected the KIS protein (arrow).
Background publications Boehm, Yoshimoto, Crook et al.: "A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression." in: The EMBO journal, Vol. 21, Issue 13, pp. 3390-401, 2002 (PubMed).

Bièche, Manceau, Curmi et al.: "Quantitative RT-PCR reveals a ubiquitous but preferentially neural expression of the KIS gene in rat and human." in: Brain research. Molecular brain research, Vol. 114, Issue 1, pp. 55-64, 2003 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN392641
291.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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